Rho-Associated Kinase Inhibitors Promote Microglial Uptake Via the ERK Signaling Pathway  

作　　者：Peicai Fu Ronghua Tang Zhiyuan Yu Caihong Li Xue Chen Minjie Xie Wei Wang Xiang Luo 

机构地区：[1]Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology [2]Key Laboratory of Neurological Diseases (HUST), Ministry of Education of China

出　　处：《Neuroscience Bulletin》2016年第1期83-98,共16页神经科学通报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(81471200 and81000521);the National Basic Research Development Program of China(2011CB504403)

摘　　要：Microglia are immunocompetent cells in the cen- tral nervous system that take up tissue debris and pathogens. Rho-associated kinase （ROCK） has been identified as an important regulator of uptake, proliferation, secretion, and differentiation in a number of cell types. Although ROCK plays critical roles in the microglial secretion of inflammatory factors, naigration, and morphology, its effects on microglial uptake activity have not been well characterized. In the present study, we found that treatment of BV2 microglia and primary microglia with the ROCK inhibitors Y27632 and fasudil increased uptake activity and was associated with morpholog- ical changes. Furthermore, western blots showed that this increase in uptake activity was mediated through the extracel- lular-signal-regulated kinase （ERK） signaling cascade, indi- cating the importance of ROCK in regulating microglial uptake activity.Microglia are immunocompetent cells in the cen- tral nervous system that take up tissue debris and pathogens. Rho-associated kinase （ROCK） has been identified as an important regulator of uptake, proliferation, secretion, and differentiation in a number of cell types. Although ROCK plays critical roles in the microglial secretion of inflammatory factors, naigration, and morphology, its effects on microglial uptake activity have not been well characterized. In the present study, we found that treatment of BV2 microglia and primary microglia with the ROCK inhibitors Y27632 and fasudil increased uptake activity and was associated with morpholog- ical changes. Furthermore, western blots showed that this increase in uptake activity was mediated through the extracel- lular-signal-regulated kinase （ERK） signaling cascade, indi- cating the importance of ROCK in regulating microglial uptake activity.

关 键 词：MICROGLIA Rho-associated kinase Uptakeactivity Extracellular-signal-regulated kinase 

分 类 号：R392[医药卫生—免疫学]