一株异养硝化-好氧反硝化细菌的分离鉴定及脱氮活性研究  

Isolation and identification of a heterotrophic nitrification-aerobic denitrification bacterium and its denitrification ability

作　　者：邹艳艳[1,2] 张宇[1] 李明智[1] 梅荣武[1] 韦彦斐[1] 丁林贤[2] 

机构地区：[1]浙江省环境保护科学设计研究院工程研究中心,浙江杭州310007 [2]浙江师范大学地理与环境科学学院,浙江金华321004

出　　处：《中国环境科学》2016年第3期887-893,共7页China Environmental Science

基　　金：浙江省环保科研计划项目(2013A006;2013B002);浙江省省属科研院所专项项目(2015F50014;2014F10011;2013F10022);浙江省公益技术研究社会发展项目(2013C33021)

摘　　要：从杭州市天子生活岭垃圾填埋垃圾渗滤液调节池周围土壤样品中分离到一株异养硝化-好氧反硝化细菌ZB612,通过形态学观察及16S r DNA同源性分析,初步鉴定属于根瘤菌属(Rhizobium sp.).随后研究了该菌株的脱氮能力,结果表明在初始氨氮浓度为100mg/L异养硝化培养基中,氨氮的去除效率达到90%,未出现明显的硝态氮和亚硝态氮积累,具有同步硝化反硝化特征;在亚硝酸盐反硝化体系中,亚硝态氮的去除效率达到60%.除此还考察了四种单因素(温度、p H值、碳氮比和碳源种类)分别对菌株ZB612脱氮效率的影响:该菌株的最佳脱氮条件为温度30oC,初始p H=7,C/N=8,以葡萄糖作为最适碳源.A bacterium ZB612 with heterotrophic nitrification-aerobic denitrification was isolated from soil sample around leachate regulating pool of Hangzhou Tianziling waste landfill. The strain ZB612 was preliminary identified as Rhizobiumsp. by morphology and 16 S r DNA homology analysis. We subsequently studied its denitrification ability, the results showed that the ammonia nitrogen removal efficiency reached 90% in the heterotrophic nitrification medium with initial ammonia nitrogen concentration of 100mg/L. Meanwhile, the obvious accumulation of nitrate-N and nitrite-N did not appeared, which is the characteristics of simultaneous nitrification and denitrification（SND）. In nitrates denitrification system,the nitrate nitrogen removal efficiency could reach 60%. In addition, we investigated the effect of four factors（including temperature, p H, carbon nitrogen ratio and carbon source） on denitrification efficiency of the strain ZB612, respectively. The optimum denitrification conditions were found to be as follows： temperature of 30℃, the initial p H of 7, C/N ratio of 8, where the optimal carbon resource was glucose.

关 键 词：根瘤菌属 异养硝化-好养反硝化 生物脱氮 同步硝化反硝化 

分 类 号：X172[环境科学与工程—环境科学]