肿瘤转移抑制基因KISS-1蛋白、基质金属蛋白酶-2和血管内皮生长因子在结肠癌组织中的表达及临床意义  

Expression and clinical significance of kisspeptin-1, matrix metalloproteinase-2 and vascular endothelial growth factor in tissue of colon cancer

作　　者：王文辉[1] 齐元玲[1] 徐谦[1] 任海朋[1] 

机构地区：[1]山东省潍坊市人民医院肿瘤内科,261042

出　　处：《中华流行病学杂志》2016年第3期415-417,共3页Chinese Journal of Epidemiology

摘　　要：目的 检测结肠癌组织中肿瘤转移抑制基因KISS-1蛋白（KISS-1）、基质金属蛋白酶-2（MMP-2）和血管内皮生长因子（VEGF）的表达,分析其与结肠癌病理特征的相关性。方法 收集2009年1月至2010年6月在潍坊市人民医院60例行结肠癌切除术患者的癌组织标本作为观察组,60例良性结肠疾病手术患者的切除组织作为对照组。采用免疫组织化学法检测KISS-1、MMP-2和VEGF的表达。结果 观察组KISS-1、MMP-2和VEGF阳性表达率分别为31.7%、58.3%和78.3%,对照组分别为73.3%、16.7%和33.3%;观察组KISS-1阳性表达率明显低于对照组,差异有统计学意义（χ^2=23.489,P〈0.001）;观察组MMP-2和VEGF的阳性表达率明显高于对照组,差异有统计学意义（χ^2=27.469,P〈0.001; χ^2=25.817,P〈0.001）。KISS-1、MMP-2和VEGF的表达与结肠癌组织学分型和TNM分期均有明显关系,差异有统计学意义（χ^2=8.997,P=0.011; χ^2=6.163,P=0.013; χ^2=8.519,P=0.014; χ^2=9.160,P=0.002; χ^2=16.577,P〈0.001; χ^2=10.523,P=0.001）。结论 KISS-1、MMP-2和VEGF检测有助于了解结肠癌分化程度和临床分期,并为判断预后和个体化治疗提供参考依据。Objective To detect the expression of kisspeptin-1 （KISS-1）, matrix metalloproteinase-2 （MMP-2） and vascular endothelial growth factor （VEGF） in the tissue of colon cancer, and analyze the relativity between KISS-1, MMP-2, VEGF and pathological characteristics of colon cancer. Methods A total of 60 colon cancer patients and 60 patients with benign colorectal disease who received surgical treatment in our hospital from January 2009 to June 2010 were selected as observation group and control group respectively. The cancer tissue samples and excision samples collected from them were used to detect KISS-1, MMP-2 and VEGF with immunohistochemistry. Results The positive rates of KISS-1, MMP-2 and VEGF were 31.7%, 58.3% and 78.3% in observation group, and 73.3%, 16.7% and 33.3% in control group. The positive rate of KISS-1 in observation group was lower than that in control group （χ^2=23.489, P〈0.001）, and the positive rates of MMP-2 and VEGF in observation group were higher than those in control group （χ^2=27.469, P〈0.001; χ^2=25.817, P〈0.001）. The expressions of KISS-1, MMP-2 and VEGF were significantly related with the histological grade and TNM stage of colon cancer （χ^2=8.997, P=0.011; χ^2=6.163, P=0.013; χ^2=8.519, P=0.014; χ^2=9.160, P=0.002; χ^2=16.577, P〈0.001; χ^2=10.523, P=0.001）. Conclusion It is helpful to understand the differentiation and clinical stage of colon cancer and provide evidence for clinical diagnosis and prognosis prediction by detecting KISS-1, MMP-2 and VEGF.

关 键 词：结肠肿瘤 肿瘤转移抑制基因KISS-1蛋白 基质金属蛋白酶-2 血管内皮生长因子 

分 类 号：R735.35[医药卫生—肿瘤]