微小RNA-451表达和胃癌临床病理特征及预后的关系  

The relations between expression quantity of microRNA -451 and clinicopathological features and prognosis in gastric cancer

作　　者：荣忠厚[1] 苏忠学[1] 耿文茂[1] 成国哲 王志意[1] 吴亚光[1] 秦成坤[1] 

机构地区：[1]山东大学附属省立医院肝胆外科,济南250021

出　　处：《中华实验外科杂志》2016年第2期318-320,共3页Chinese Journal of Experimental Surgery

基　　金：山东省科技厅科技发展计划基金（2013GSF11862）

摘　　要：目的探讨微小RNA-451（miR-451）的表达与胃癌的临床病理特征及预后之间的关系。方法收集107例胃癌患者的病理及随访资料，应用实时定量反转录聚合酶链反应（RT-qPCR）方法检测肿瘤标本及癌旁组织的miR-451的表达量。然后分析miR-451的表达量和胃癌患者的各项病理特征，及预后之间的关系。结果与癌旁组织比较，胃癌组织内miR-451表达量降低（0．010±0．007比0．014±0．008，P〈0．05）；与无淋巴结转移的胃癌患者比较，淋巴结转移者癌组织miR-451表达量降低（0．008±0．006比0．013±0．006，P〈0．05）；与miR-451表达上调的胃癌患者比较，miR-451表达下调者生存时间缩短（中位生存时间45个月比60个月，P〈0．05）。多因素分析表明，miR-451的低表达与胃癌患者淋巴结转移及不良预后相关。结论miR-451的低表达与胃癌患者淋巴结转移及不良预后相关。Objective To investigate the relations between expression of microRNA-451 （ miR -451 ） and clinicopathological features and prognosis of patients in gastric cancer. Methods Pathological data and follow up data of 107 cases in gastric cancer were collected, expression of miR -451 in tumor tissue and pericarcinous tissue was measured by real -time quantitative reverse transcriptase -polymerase chain reaction （RT - qPCR ）. After that, we analyzed the relations between expression of miR -451 and clinicopathological features and prognosis; Results MiR -451 showed decreased expression in tumor tissue versus pericarcinous tissue （ 0. 010± 0. 007 vs. 0, 014 ± 0, 008, P 〈 0.05 ） ; miR -451 also showed decreased expression in tumor tissue of the patients with lymphatic metastasis versus the patients without lymphatic metastasis （0. 008 ±0. 006 vs. 0. 013 ±0. 006, P 〈0. 05） ; down - regulation of miR - 451 in the patients showed shorter survival time （ median survival time 45 months vs. 60 months, P 〈 0. 05 ） ; multivariate analysis revealed that down - regulation of miR - 451 was associated with lymphatic metastasis and worse prognosis of the patients in gastric cancer. Conclusion Down - regulation of miR -451 was related to lymphatic metastasis and worse prognosis of the patients in gastric cancer.

关 键 词：胃癌 微小RNA-451 

分 类 号：R735.2[医药卫生—肿瘤]