Apelin-13对兔脑外伤模型中自噬相关蛋白表达中的影响及其机制  

Effect of Apelin - 13 on autophagy - associated protein expression in rabbit cerebral cortex after traumatic brain injury

作　　者：刘继新[1] 依斯哈尔·巴拉提 王志丹[1] 李文娟[1] 李春丽[1] 迪里夏提·依马木 

机构地区：[1]新疆维吾尔自治区人民医院北院神经外科,乌鲁木齐830054

出　　处：《中华实验外科杂志》2016年第2期400-403,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察Apelin-13对兔脑外伤自噬相关蛋白的影响，探讨其对兔脑外伤后自噬相关蛋白调节的作用机制。方法取健康雄性新西兰大白兔30只，随机分为假手术（Sham）组、生理盐水（Saline）组、Apelin-13组。Apelin-13组和Saline组在建立脑外伤模型前10min，分别注射Apelin-13（0．3μg／g体质量）或等量生理盐水于侧脑室，然后利用Western blot检测脑外伤后24、48h组大脑皮质自噬相关蛋白微管相关蛋白1轻链3（LC3）、p62、Beclin-1和B细胞淋巴瘤／白血病-2（bcl-2）等的表达。结果与Sham组比较，在脑外伤后24、48h，Saline组自噬相关蛋白LC3—Ⅱ／LC3-I比值（1．147±0．125），Beclin-1的蛋白表达（1．035±0．112），均显著上调（P〈0．01），同时bcl-2、p62的蛋白表达水平（0．613±0．087、0．608±0．085）显著下调。然而，与Saline组比较，Apelin-13组可显著下调自噬相关蛋白LC3-Ⅱ／LC3-1比值（0．226±0．048）和Beclin-1的蛋白表达水平（0．181±0．039，P〈0．01），同时显著上调bcl-2和p62的表达水平（0．385±0．062和0．360±0．035），亦明显降低Beclin-1／bcl-2的比值（0．704±0．064）。结论Apelin-13可以明显抑制脑外伤后兔模型大脑皮质细胞的自噬活性，这一作用可能是通过调节Beciin-1、bcl-2蛋白表达水平来实现的。Objective To observe the effect of Apelin - 13 on rabbit traumatic brain injury （TBI）, and explore the relationship between Apelin - 13 and autophagy in TBI. Methods Thirty New Zealand white rabbits were randomly divided into sham operation group, saline group and Apelin - 13 group. Apelin - 13 and saline groups were pretreated with an injection of Apelin - 13 （0. 3 μg/g） and saline into the rabbit brain lateral ventricle respectively 10 min before TBI. The autophagy - associated proteins microtubule - associated protein 1 light chain 3 （ LC3）, p62, Beclin - 1 and B cell lymphoma/leuke- mia - 2 （ bcl - 2 ） in the rabbit cerebral cortex were assessed by Western blotting 24 h and 48 h after TBI. Results As compared with Sham group, protein LC3 - Ⅱ/LC3 - Ⅰ ratio and Beclin - 1 expression were up -regulated （1. 147± 0. 125 and 1. 035± 0. 112） , while protein bcl -2 and p62 were downregulated in saline group 24 h and 48 h after TBI （0. 613 ±0. 087 and 0. 608 ±0. 085）. As compared with saline group, Apelin - 13 decreased protein LC3 - Ⅱ/LC3 - Ⅰ ratio and Beclin - 1 expression （0. 226±0. 048 and 0. 181 ±0. 039）, increased protein bc1-2 and 1362 expression （0. 385±0. 062 and 0. 360±0. 035）, and downregulated protein Beclin -1/bcl -2 ratio after TBI （0. 704 ± 0. 064 ）. Conclusion Apelin- 13 suppresses autophagy in rabbit TBI models possibly by down - regulating protein Beelin - 1/bel - 2 ratio.

关 键 词：APELIN-13 脑外伤 自噬相关蛋白 兔 

分 类 号：R363[医药卫生—病理学]