大豆抗胞囊线虫4号小种转录组测序及分析  

Transcriptome Sequencing and Analysis of Soybean(Glycine max)Resistance to Race 4 of Soybean Cyst Nematode(Heterodera glycines)

作　　者：吴书峰[1,2] 王志[2] 乔治军[2] 张瑞军[2] 张海平[2] 

机构地区：[1]山西大学生物工程学院,太原030006 [2]山西省农业科学院农作物品种资源研究所,农业部黄土高原作物基因资源与种质创制重点实验室,太原030031

出　　处：《农业生物技术学报》2016年第4期519-529,共11页Journal of Agricultural Biotechnology

基　　金：山西省科技厅自然基金(No.2015011073);农业部作物种质资源保护子项目(No.2015NWW030-14-21);山西省农业科学院博士后项目(No.BHS-2015JJ-001)

摘　　要：大豆胞囊线虫(soybean cyst nematode,SCN)(Heterodera glycines)是一种世界性的大豆(Glycine max)病害。本研究以抗SCN 4号生理小种的黑豆为研究对象,对大豆胞囊线虫侵染后的两个发育时期进行转录组测序和生物信息学分析,宏观了解大豆的抗病机制。通过Illumina Hi SeqTM2500测序共获得1.96×1010bp的数据。经过分析后,接种后第9天(第一时期)和接种后第17天(第二时期)分别得到2 180个和4 210个差异表达基因,同时,对差异表达基因进行基因本体(Gene Ontology,GO)注释,蛋白质直系同源簇(Clusters of Orthologous Groups of proteins,COG)注释,结果显示,GO注释和COG注释分别将差异表达基因分为了58和25个功能类别。另外,京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析结果表明,可将两个时期的差异表达基因分别定位到83和105个具体的代谢途径分支。其中,参与到激素信号转导途径,苯丙氨酸代谢,能量代谢等过程中的差异基因最多,这些数据为进一步筛选抗病关键基因及功能验证等研究提供了依据。Soybean cyst nematode （SCN）（Heterodera glycines） is the most devastating pathogen of soybean （Glycine max） in the world. To complete the life cycle, SCN enters into host roots and causes dramatic morphological and physiological changes in host ceils. At the same time, the gene＇s expression changed in host roots. In this paper, Illumina HiSeqTM 2500 technique was used to sequence the genes expression of the soybean, which was resistance to race 4 of SCN, after SCN infection 9 and 17 d. These time points were chosen to contrast with plant gene expression without inoculated cyst nematode. The previous time point was identified because it was the development transition period of second-stage juvenile （J2） to third-stage juvenile （J3）. Similarly, the later time point was the development period of third-stage juvenile （J3） to the fourth-stage juvenile 04）. Finally, a total of 1.96× 1010 bp bases were obtained. There were 2 180 and 4 210 genes with altered expression in roots at 9 day and 17 day after SCN infection, respectively, and a total of 840 common genes expressed between 2 periods. Gene Ontology （GO） significant enrichment analysis displayed 1 898 and 3 627 differentially expressed genes （DEG） which could be divided into cellular component, molecular function and biological processes of these 3 ontology. And there was relatively more DEG classified to extracellular region, antioxidant activity, nutrient reservoir activity and cell killing. Differentially expressed genes were classified into 25 categories using Cluster of Orthologous Groups of proteins （COG） annotation, and a total of 880 and l 751 DEG annotated to this database at those 2 periods respectively. The function of COG was more comprehensive, the general function prediction only had the largest proportion, and the second was the transcription. In addition, gene expression patterns were integrated with biochemical pathways from the Kyoto Encyclopedia of Genes and Genomes （KEGG）. Part of DEG might be involved

关 键 词：大豆 大豆胞囊线虫(SCN) 转录组测序 功能注释 

分 类 号：S565.1[农业科学—作物学] Q943.2[生物学—植物学]