慢性牙周炎患者龈沟液中EMMPRIN及其配体CyPA的表达及意义  

Expressions of EMMPRIN and its ligand CyPA in gingival crevicular fluid of chronic periodontitis patients

作　　者：何艳萍[1,2] 谢明[1] 焦婷[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科,上海市口腔医学重点实验室,上海200011 [2]同济大学附属东方医院口腔科,上海200126

出　　处：《上海口腔医学》2016年第1期27-31,共5页Shanghai Journal of Stomatology

基　　金：上海市科学技术委员会资助项目(114119a3900,14411964200)

摘　　要：目的:通过检测慢性牙周炎患者龈沟液中EMMPRIN及其配体CyPA的表达,探索两者与牙周炎症的关系。方法:收集慢性牙周炎患者(牙周炎组)以及无缺牙、无牙周炎患者(对照组)的天然牙龈沟液,并进行临床检查。以ELISA法检测龈沟液中EMMPRIN及CyPA的表达,应用SPSS 17.0软件包分析两者与牙周炎的关系,比较2组之间的差异。结果:牙周炎组中,GCF总量与EMMPRIN总量、CyPA总量以及GI、SBI、AL呈显著正相关;EMMPRIN总量与GCF总量、CyPA总量以及GI、SBI、AL呈显著正相关,与吸烟情况呈负相关(P<0.05);CyPA总量与GCF总量、EMMPRIN总量以及GI、SBI、AL呈显著正相关。对照组中,GCF、EMMPRIN及CyPA三者总量两两间均呈显著正相关。在牙周炎组与对照组之间,GCF、EMMPRIN及CyPA三者总量的组间差异均有统计学意义(P<0.05)。结论:EMMPRIN及其配体CyPA在牙周健康患者、慢性牙周炎患者的龈沟液中均有表达,当牙周炎症临床症状较重时,分泌的龈沟液量明显增多,龈沟液中EMMPRIN及CyPA的表达也增多。PURPOSE： To detect the expressions of EMMPRIN and its ligand CyPA in gingival crevicular fluid （GCF） of chronic periodontitis （CP） patients and explore their possible relation to the status of periodontal inflammation. METHODS： GCF of CP patients （group CP） and periodontitis-free patients with intact dentition （the control group） were collected and assayed for EMMPRIN and CyPA expressions by ELISA. The clinical periodontal status of these patients were examined. Statistical analysis was performed by use of SPSS 17.0 software package. Spearman＇s correlation analysis was utilized to determine the relationships between the expressions of EMMPRIN and CyPA in GCF and the clinical parameters. In addition, analysis of variance （ANOVA） was used for comparing the difference between group CP and the control group. RESULTS： In group CP, GCF volume was positively correlated with EMMPRIN total amount, CyPA total amount and some clinical periodontal indexes （GI,SBI,AL）. EMMPRIN total amount was positively correlated with GCF volume, CyPA total amount and some of clinical periodontal indexes （GI,SBI,AL）, but it was negatively correlated with smoking status （P〈0.05）. Moreover, CyPA total amount was positively correlated with GCF volume, EMMPRIN total amount and some of clinical periodontal indexes （GI,SBI,AL）. In the control group,there were significant positive correlations among GCF volume, EMMPRIN total amount and CyPA total amount. The difference of GCF, EMMPRIN and CyPA between the 2 groups were statistically significant （P〈0.05）. CONCLUSIONS： EMMPRIN and its ligand CyPA in GCF of periodontitis-free patients with intact dentition and CP patients were all detected. As the progress of periodontal inflammation, GCF secretion increases, as well as the expressions of EMMPRIN and CyPA in GCF.

关 键 词：细胞外基质金属蛋白酶诱导因子 亲环素A 龈沟液 慢性牙周炎 

分 类 号：R781.4[医药卫生—口腔医学]