姜科植物益智儿茶素含量测定分析研究  

Study on Determination of The Content of Catechin in Alpinia oxyphylla

作　　者：田建平[1] 胡远艳[2] 王俊芳[1] 

机构地区：[1]海南医学院药学院,海南海口571199 [2]海南大学热带生物资源教育部重点实验室,海南海口570228

出　　处：《广州化工》2016年第5期149-151,共3页GuangZhou Chemical Industry

基　　金：海南医学院科研培育基金(HY2012-024)

摘　　要：对益智不同部位和储藏年份中儿茶素含量进行了研究。利用超声提取和紫外分光光度法,在检测波长280 nm处测定样品吸光度,计算儿茶素含量。结果表明:提取儿茶素最适宜的条件为80%乙醇、1∶10料液比,超声提取时间40 min;平均加样回收率为92.5%,RSD=0.074%(n=5);益智不同部位的儿茶素含量依次为种子(0.50%)>叶(0.49%)>茎(0.21%)>果皮(0.06%);不同储藏年份的益智果实儿茶素含量依次为2014年产(0.48%)>2013年产(0.46%)>2012年产(0.36%)。益智叶为一类值得开发的药用资源;同时应大力改善储藏条件,以减少益智有效成分含量的损失。The content of Catechin in different parts of Alpinia oxyphylla and the content of the Catechin in different storage years of fruit of A. oxyphylla were studied. Ultrasonic extraction and ultraviolet spectrophotometric methods were used,and detection wavelength was in 280 nm. Results showed that the most suitable conditions were 80% ethanol,1∶10ratio,40 min of ultrasonic extraction time,the average recovery rate was 92. 5%,RSD = 0. 074%（ n = 5）. The content of catechin in different parts of A. oxyphylla was seed（ 0. 50%）〉 leaf（ 0. 49%） 〉stem（ 0. 21%） 〉pericarp（ 0. 06%）,and the content of fruit in different storage years was 2014（ 0. 48%） 〉2013（ 0. 46%）〉 2012（ 0. 36%）. Leaf of A. oxyphylla was a kind of medicinal resources worth developing,and the storage conditions should be improved,so as to reduce the loss of the content of the effective components of fruit of A. oxyphylla.

关 键 词：益智 儿茶素 含量测定 

分 类 号：R931.6[医药卫生—生药学]