麦胚肽(RVF)对人神经瘤细胞SH-SY5Y损伤模型的保护作用  

Protective Effect of Wheat Germ Peptide on Cultured SH-SY5Y Human Neuroblastoma Cell Line in an in vitro Cell Injury Model

作　　者：程云辉[1] 吕兆良 孙威[1] 唐季清 许宙[1] 文李[1] 俞惠新[2] 张莉[2] 

机构地区：[1]长沙理工大学化学与生物工程学院,湖南长沙410114 [2]江苏省原子医学研究所,江苏无锡214063

出　　处：《现代食品科技》2016年第1期13-21,共9页Modern Food Science and Technology

基　　金：国家自然科学基金项目(31171627;31071523);"十二五"国家科技支撑计划(2012BAD34B02)

摘　　要：本文研究了麦胚肽(RVF)对人神经瘤细胞SH-SY5Y损伤的保护作用。采用体外细胞培养法,以人神经瘤细胞SH-SY5Y为材料,建立了饥饿损伤和H2O2损伤模型。结果表明:在40个不同浓度与作用时间的组别中,对SH-SY5Y细胞具有显著营养功能的只有无血清饥饿模型中200μmol/L RVF作用12 h与含1%血清饥饿模型中500μmol/L RVF作用24 h两个组别,且不具有剂量与时间依赖性。在H2O2诱导的细胞氧化损伤模型中,200μmol/L的RVF将细胞存活率分别提高了25.05%(MTT法)和21.90%(SRB法);200μmol/L RVF预孵育时间延长至12 h时,RVF极显著地提高了细胞存活率,且具有剂量与时间依赖性;细胞形态检测结果表明RVF干预后,SH-SY5Y细胞也逐渐由皱缩、细长恢复到梭形或多角形,粘附性增强。在本研究建立的两种损伤模型中,RVF仅对H2O2损伤模型具有显著保护作用。The protective effects of wheat germ peptide（RVF） on the SH-SY5 Y human neuroblastoma cell line were investigated in this study. Starvation- and hydrogen peroxide（H2O2）-induced injury models were established by in vitro cell culturing of an SH-SY5 Y human neuroblastoma cell line. The SH-SY5 Y cells were treated with 40 RVFs at different concentrations and incubation times. The results showed significant nutritional functions only in the group pre-incubated with 200 μmol/L RVF for 12 h in a no-serum starvation-induced injury model and the group treated with 500 μmol/L RVF for 24 h in the 1% serum starvation-induced injury model, and no dose- or time-dependent relationships were found. In the H2O2-induced injury model, treatment with 200 μmol/L RVF increased cell survival rates by 25.05 and 21.90% in the methyl-tetrazolium（MTT） and sulforhodamine B（SRB） assays, respectively, in a time- and dose-dependent manner. The cell morphological analysis indicated that after RVF treatment, the SH-SY5 Y cells gradually resumed a spindle or polygonal shape from a wrinkled, shrunken, and elongated shape, with enhanced cell adhesion. In the two injury models investigated in this study, RVF only showed a significant protective effect in the H2O2-induced injury model.

关 键 词：麦胚肽 SH-SY5Y 饥饿 氧化损伤 

分 类 号：TS201.4[轻工技术与工程—食品科学]