SARS-CoV、SARSL-CoV与其他冠状病毒的种系发生分析  

Phylogenetic analysis of SARS-CoV,SARSL-CoV,and other coronavirus

作　　者：朱静媛[1] 李庆波[1] 丁记者 何剑锋[2] 徐德忠[3] 段广才[1,4] 

机构地区：[1]郑州大学公共卫生学院环境卫生学教研室,河南郑州450001 [2]广东省疾病预防控制中心传染病预防控制所,广东广州511430 [3]第四军医大学预防系流行病学教研室,陕西西安710032 [4]新乡医学院河南省分子诊断与医学检验技术协同创新中心,河南新乡453003

出　　处：《中华疾病控制杂志》2016年第2期107-111,128,共6页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金(81373059)

摘　　要：目的分析不同流行时段重症急性呼吸综合征冠状病毒(severe acute respiratory syndromes coronavirus,SARS-CoV)病毒株与重症急性呼吸综合征样冠状病毒(severe acute respiratory syndromes like coronavirus,SARSLCoV)及其他冠状病毒的分子进化关系。方法从美国国立生物技术信息中心获取不同分离时段的SARS-CoV(9条)、所有SARSL-CoV(24条)、其他冠状病毒(26条)的全基因组序列,用Clustal X 2.1软件进行多重序列比对,用邻接法(neighbor-joining,NJ)MEGA 6.0软件构建系统进化树。结果在包含SARS-CoV、SARSL-CoV和其他冠状病毒基因组序列的不同数据集中,流行早、中期SARS-CoV与流行末期毒株的基因组序列均分别属于不同的分支;以流行早、中期SARS-CoV为根的有根树显示,流行末期SARS-CoV遗传距离最远,而与SARSL-CoV和其他冠状病毒遗传距离更接近。结论流行末期SARS-CoV与流行早、中期SARS-CoV遗传差异大,且可能存在逆向/返祖进化。Objective To analyze the molecular evolution of severe acute respiratory syndromes coronavirus（ SARS-CoV） genomic sequences in different epidemic phase and to determine their possible relationship with SARS-like CoV or other coronavirus. Methods Nine SARS-CoV genomic sequences derived from different epidemic phases,24 SARS-like CoV and 26 other coronavirus genomic sequences were collected. Multiple sequence alignments were conducted by Clustal X2. 0 and the phylogenetic trees were constructed using MEGA 6. 0 software through Neighbor-Joining（ NJ）method. Results SARS-CoV genomic sequences derived from early and middle phases were attributed to one branch and those from late phase to another separated branch with far genomic distance in every phylogenetic trees. With root of SARSCoV genomic sequences derived from early and middle phases,those derived from late phases were the furthest branch and closer to SARSL-CoV or other coronavirus genomic sequences. Conclusions SARS-CoV genomic sequences derived from late phase have great genetic differences compared with those derived from early and middle phases. It＇s suggested that reverse or atavistic evolution occurred during the late phase of SARS epidemic.

关 键 词：SARS病毒 冠状病毒科 系统分析 

分 类 号：R394.3[医药卫生—医学遗传学] R181[医药卫生—基础医学]