早期食管鳞癌与正常食管组织中微小RNA差异性表达的分析  

Analysis of differentially expressed mi RNA in early esophageal squamous cancer and normal esophageal tissues

作　　者：郝志鹏[1] 唐朗[1] 王鹏[1] 邓豫[1] 蔡奕欣[1] 张霓[1] 李维娜[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院胸外科,武汉市430030 [2]华中科技大学同济医学院附属同济医院感染科,武汉市430030

出　　处：《实用医学杂志》2016年第4期523-526,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81301505);华中科技大学前沿探索基金项目(编号:2015TS140)

摘　　要：目的:分析早期食管鳞癌和正常食管组织的微小RNA(miRNAs)表达谱,找出具有显著表达差异的miRNAs并研究其对EC109增殖的影响。方法:微阵列技术(microarray)检测3对早期食管鳞癌和对应正常食管组织中miRNAs表达谱并进行分析,qRT-PCR法在另38对样本中对有显著表达差异的miRNAs进一步验证;将验证确定的miR-221、miR-222和miR-29a的模拟物(mimics)分别转染至食管鳞癌细胞EC109中,CCK8法研究高表达各miRNAs后对细胞增殖的影响。结果:微阵列技术共筛出53个与早期食管鳞癌相关的差异表达miRNAs,32个表达上调,21个表达下调;qRT-PCR法证实早期食管鳞癌中miR-29a的表达显著降低(P<0.05),而miR-221和miR-222的表达显著升高(P<0.05)。在EC109中高表达miR-29a可抑制细胞增殖(P<0.05),而高表达miR-221和miR-222均能促细胞增殖(P<0.05)。结论:早期食管鳞癌和正常食管组织间存在miRNAs的表达差异,这些差异性表达的miRNAs可能成为食管鳞癌早期诊断的新靶点。Objective To investigate the expression profiles of microRNA in early esophageal squamous cancer and noimal esophageal tissues and verify the significantly different expression miRNAs, further to study the effects on proliferation of EC109. Methods The microarray assay was performed to analyze miRNA expression profiles in three pairs of early esophageal squamous cancer and the corresponding normal esophageal tissues. Quantitative real-time polymerase chain reaction （qRT-PCR） was used in another 38 pairs samples to further verify the differentially expressed miRNAs . Three verified miRNAs （ miR-29a, miR-221 and miR-222） mimics were transfected into EC109 respectively and CCK8 method was used to study the effect of cell proliferation in each miRNA. Results Microarray technique selected 53 miRNAs that differentially expressed in early esophageal squamous cancer and normal esophageal tissues, 32 miRNAs were up-regulated and 21 miRNAs were down-regulated, qRT-PCR verified that miR-29a was significantly down-regulated（P 〈 0.05） and miR-221, miR-222 were significantly up-regulated （P 〈 0.05 ） in early esophageal squamous cancer tissue. Over-expression of miR-29a could significantly inhibit the proliferation of EC109（P 〈 0.05） whereas over-expression of miR-221 or miR-222 could both significantly promote the proliferation of EC109 （P 〈 0.05）. Conclusion There was significant difference of miRNAs expression between early esophageal squamous cancer and normal esophageal tissues, and the differentially expressed miRNAs could be used as new biomarkers for early diagnosis of esophageal squamous cancer.

关 键 词：食管鳞癌 微小RNA 细胞增殖 早期诊断 

分 类 号：R735.1[医药卫生—肿瘤]