1803例女性人乳头瘤病毒基因分型检测及感染情况分析  

Analysis of 1803 Cases of Female Human Papilloma Virus Genotype and Infection

作　　者：李世君[1] 王芳[1] 

机构地区：[1]赤峰市医院病理科,内蒙古赤峰024000

出　　处：《内蒙古医学杂志》2016年第1期46-48,共3页Inner Mongolia Medical Journal

摘　　要：目的了解赤峰地区女性宫颈人乳头瘤病毒（HPV）感染的阳性率及各亚型的分布情况,为本地区宫颈癌的防治提供依据。方法采用聚合酶链反应（PCR）体外扩增和反向斑点杂交基因芯片技术,检测2014年8月至2015年8月在本院就诊的1 803例女性的宫颈脱落细胞标本。结果1 803例标本中HPV阳性感染547例,总感染率为30.34%。26种亚型中共检出25种亚型,其中低危亚型HPV57型未检出,高危亚型组优势型别依次为HPV16、58和51型,低危亚型组以HPV54型为主。单一亚型占总感染的71.12%,多重亚型占总感染的28.88%。HPV高危亚型组占阳性标本的73.13%,HPV低危亚型组占阳性标本的13.89%,混合感染组占阳性标本的12.98%。HPV感染的高峰年龄为36-55岁,各年龄组的HPV阳性检出率差异有统计学意义（P〈0.01）。结论HPV基因分型检测对本地区宫颈癌的筛查、防治具有重要的指导意义。Objective To investigate the positive rate of female HPV infection and the distribution of each gene subtype in Chifengregion,in order to provide the basis for the prevention and control of cervical cancer.Methods Polymerase chain reaction（PCR）and reverse clot blot hybridization and DNA microarray technology were used to detect 1803 cases of female cervical cytology specimens that collected from August2014 to August 2015.Results There were 547 cases of positive HPV in 1803 samples,and the positive rate was 30.34%.25 kinds of subtypes were detected in26 kinds of knownsubtypes.The low-risk type HPV-57 had not been detected in this study.High-risk superior types were HPV16,58,51 and low-risk type was mainly HPV54 subtype.Single HPV subtype infection rate was 71.12%,and multiple HPV subtype infection rate was 28.88%,The infection rate of high-risk type and low-risk type were 73.13%and13.89%in positive HPV samples.The peak age of HPV infection ranged from 36 to 55 years old.There was significant difference between aged groups in HPV positive detection rate（P〈0.05）.Conclusion HPV genotyping detection is meaningful in cervical cancer prevention and treatment in local area.

关 键 词：人乳头瘤病毒 基因分型 宫颈癌 

分 类 号：R73[医药卫生—肿瘤]