野生樱桃李叶总黄酮含量测定方法比较研究  

Comparison of the Determination Methods for Total Flavonoids in Prunus divaricata Ldb Leaves

作　　者：刘伟[1] 高红艳[1] 李紫薇[1] 腊萍[1] 万春鹏[2] 

机构地区：[1]伊犁师范学院新疆维吾尔自治区普通高校天然产物化学与应用重点实验室,新疆伊宁835000 [2]江西农业大学农学院/江西省果蔬保鲜与无损检测重点实验室,江西南昌330045

出　　处：《江西农业大学学报》2016年第1期192-197,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：自治区教育厅普通高校重点实验室资助项目(2013YSHXZD03)~~

摘　　要：采用高效液相色谱法、NaNO_2-Al(NO_3)_3法、AlCl_3法测定野生樱桃李叶总黄酮含量。结果表明,高效液相色谱法测定总黄酮含量为(36.53±0.46)mg/g,Al Cl_3法测定总黄酮含量为(38.27±0.59)mg/g,两种方法测定结果差异不显著;而NaNO_2-Al(NO_3)_3法测定总黄酮含量为(49.81±0.70)mg/g,与其它两种方法测定结果差异较大。通过显色机理分析知AlCl_3法可消除干扰,重现性好,准确度高,操作相对高效液相色谱法更快捷简便,确定AlCl_3法为测定野生樱桃李叶总黄酮含量的最佳方法。The total content of flavonoids in Prunus divaricata Ldb Leaves was determined by HPLC method,aluminum nitrate-sodium nitrite method and aluminum chloride method. The results showed that the total content of flavonoids in Prunus divaricata Ldb Leaves was（ 36. 53 ± 0. 46） mg / g and（ 38. 27 ± 0. 59） mg / g by HPLC method and aluminum chloride method,respectively.The difference of the results determined by the two methods was not significant（ P〉0.05）.However,the total content of flavonoids determined by aluminum nitrate-sodium nitrite method was（ 49.81 ± 0.70） mg / g,which rather different from the was results of the other two methods.The analysis on mechanism of color development indicated that the aluminum chloride method can eliminate the interference.This method is simpler and more convenient compared with HPLC method,and has high accuracy and repeatability,and is the most appropriate for the determination of total flavonoids in Prunus divaricata Ldb Leaves.

关 键 词：野生樱桃李 总黄酮 分光光度法 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]