大鼠局灶性脑缺血再灌注后海马CA1区TASK-1表达及其与星形胶质细胞活化的关系  

Changes in TASK-1 Expression and Its Involvement in Astrogliosis Following Cerebral Ischemia in Rat CA1 region of Hippocampus

作　　者：袁建清[1] 钟善全[1] 刘铮[1] 廖春英[1] 

机构地区：[1]赣南医学院第一附属医院神经内科,江西赣州341000

出　　处：《赣南医学院学报》2016年第1期24-26,共3页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：目的:观察一种新型双孔钾离子通道亚单元TASK-1在大鼠海马CA1区的表达及其在局灶脑缺血再灌注后的动态变化,探讨TASK-1表达与星形胶质细胞活化的关系。方法:建立大鼠大脑中动脉阻塞(MCAO)再灌注模型,采用免疫荧光组化和激光扫描共聚焦观察大鼠MCAO再灌注后3、7、28 d海马CA1区TASK-1和GFAP表达。结果:成年大鼠海马CA1区可见大量TASK-1和GFAP双标阳性的细胞,MCAO再灌注后3、7、28 d海马CA1区TASK-1和GFAP表达逐步上调,与假手术组比较均有显著差异(P<0.05)。结论:脑缺血再灌注后大鼠海马CA1区TASK-1的表达上调与星形胶质细胞的活化有一定关系。Objective： To investigate changes in TASK-1 expression and its involvement in astrogliosis following cerebral ischemia in rat CA1 region of hippocampus. Methods： Focal ischemia was induced by temporary middle cerebral artery occlusion（ MCAO）. The experimental rats induced by MCAO were sacrificed on days 3,7 and 28 after reperfusion. The expressions of TASK-1 and GFAP on astrocytes in the CA1 region of Hippocampus were detected by immunofluorescence and confocal microscope. Results： TASK-1 was broadly expressed on astrocytes in the CA1 region of hippocampus. After MCAO,the expressions of both TASK-1 and GFAP on astrocytes were significantly increased at days 3,7,28 following reperfusion. Conclusion： After MCAO,the increased TASK-1 expression on astrocytes in rat CA1 region of hippocampus is related with astrocyte activation.

关 键 词：TASK-1通道 星形胶质细胞 海马CA1区 脑缺血 

分 类 号：R743[医药卫生—神经病学与精神病学]