卵巢癌患者外周血调节性T细胞的变化及其临床意义  

Regulatory T cell levels in peripheral blood of patients with ovarian cancer and its clinicopathological significance

作　　者：吴杰[1] 骆骥才[2] 陈文虎[1] 许志良[3] 张剑英[1] 张毅敏[1] 金元[1] 刘鹏[1] 徐笑红[1] 

机构地区：[1]浙江省肿瘤医院检验科,杭州310022 [2]浙江大学医学院附属第一医院检验科 [3]中国科学院(杭州)生命科学院

出　　处：《浙江医学》2016年第4期245-247,259,共4页Zhejiang Medical Journal

基　　金：浙江省卫生厅资助项目(2015KYA042)

摘　　要：目的探讨卵巢癌患者外周血调节性T细胞(Treg)水平变化的意义及其临床意义。方法采用流式细胞术测定130例卵巢癌患者和50例体检健康女性的外周血中CD4^+、CD8^+、NK以及Treg等不同淋巴细胞亚群的比例,比较两组间的差异,分析卵巢癌不同临床病理因素患者Treg水平的差异,并比较手术和化疗患者手术前后或化疗前后Treg水平的变化。结果卵巢癌组外周血CD4^+、NK细胞比例以及CD4^+/CD8^+比值均明显低于正常对照组,而Treg细胞比例明显高于正常对照组;淋巴结转移卵巢癌患者其Treg水平高于淋巴结未转移,且在不同的FIGO分期中Ⅲ、Ⅳ期患者的Treg细胞比例要高于Ⅰ、Ⅱ期,差异均有统计学意义(均P<0.05);手术后Treg细胞比例较手术前明显下降,差异有统计学意义(P<0.05);化疗后Treg细胞比例与化疗前的差异无统计学意义(P>0.05)。结论卵巢癌患者外周血Treg水平与卵巢癌的分期和进展有关,在卵巢癌预后判断以及疗效监测中有重要意义。Objective To investigate the levels of CD4＋CD25＋CD1271ow regulatory T cells in peripheral blood of patients with ovarian cancer and its relation to ciinicopathological features of the disease. Methods Plasma levels of CD4, CD8, NK and regulatory T cells were measured in 130 ovarian cancer patients and 50 healthy controls by flow cytometry. The relation between the plasma levels of regulatory T cells and clinicopathologic features of the disease was analyzed, and changes of regulatory T cells levels in 30 ovarian cancer patients before and after surgery and chemotherapy were compared. Results The percentages of CD4~, NK and CD4^＋/CD8^＋ ratio in ovarian cancer group were significantly lower than those in control group. The percentage of regulatory T cells in ovarian cancer group was significantly higher than that in controls; the percentage of regulatory T cells in patients with lymphatic metastasis was significantly higher than that in those without lymphatic metastasis; and the percentage of regulatory T cells in advanced cancer patients was significantly higher than that in early cancer patients （P〈0.05）. The levels of regulatory T cells dropped significantly after surgery （P〈0.05）, but had no significant changes after chemotherapy （P 〉0.05）. Conclusion The results indicate that regulatory T cells may be a valuable marker for prognosis and treatment monitoring in patients with ovarian cancer.

关 键 词：调节性T细胞 卵巢癌 流式细胞术 

分 类 号：R737.31[医药卫生—肿瘤] R730.43[医药卫生—临床医学]