中药膏剂呼吸道给药对S180荷瘤小鼠肿瘤生长及免疫功能的影响  

Effects of Chinese medicine on tumor inhibition and immune function in S180 tumor-bearing mice

作　　者：巨英超[1] 刘亮[2] 

机构地区：[1]河北医科大学第四医院实验动物中心,石家庄050011 [2]河北医科大学第四医院肿瘤所,石家庄050011

出　　处：《解放军医学杂志》2016年第3期195-198,共4页Medical Journal of Chinese People's Liberation Army

基　　金：河北省医学科学研究重点课题(ZL20140126);河北省普通高等学校强势特色学科肿瘤学建设经费[冀教高(2005)52号]~~

摘　　要：目的研究中药膏剂通过呼吸道给药对S180荷瘤小鼠肿瘤的抑制作用及对其免疫功能的影响。方法将S180细胞接种至昆明小鼠腹腔,待形成腹水后,取腹水接种至昆明小鼠左前肢皮下,建立皮下移植瘤模型,待瘤体体积达0.06cm3时开始用药。实验分为3组,每组6只。对照组不给予任何干预措施,正常饲养。中药组经呼吸道给药治疗,每日通过呼吸道吸入药物气体6h,1次/d,持续给药21d。阿霉素(ADM)组经腹腔注射ADM 1mg/kg,每周1次,共3次。每日观察昆明小鼠的进食、活动状况等一般体征,隔日测量小鼠体重。停药2d后,经小鼠内眦静脉取血,采用流式细胞术检测CD3^+、CD4^+和CD8^+T淋巴细胞比例。剥离小鼠皮下瘤结节称重,部分瘤组织进行病理学观察,部分制备成单细胞悬液,采用流式细胞术检测细胞凋亡情况。结果与对照组相比,中药组小鼠静脉血中CD3^+、CD4^+T淋巴细胞比例和CD4^+/CD8^+比值明显增高(P<0.05)。与对照组比较,中药组和ADM组小鼠皮下移植瘤重量明显降低(P<0.01),而中药组与ADM组皮下移植瘤的重量差异无统计学意义(P>0.05)。与对照组比较,中药组细胞凋亡率明显增加(P<0.05)。镜下病理学观察可见对照组移植瘤组织坏死局限,细胞丰富,排列紧密,中药组瘤组织内坏死广泛分布,无明显区域性,纤维组织增生,瘤细胞散在排列,瘤组织周围淋巴细胞浸润较多。ADM组瘤组织内亦可见较多坏死组织。结论中药膏剂经呼吸道吸入给药可在一定程度上抑制小鼠S180皮下移植瘤的生长。Objective To study the inhibitory effect of the Chinese medicine on S180 tumor-bearing mice and the effect on the immune function of the mice by respiratory drug administration. Methods The S180 cells were inoculated into the abdominal cavity of Kunming mice. After the ascites was formed, the left anterior limb of mice was inoculated with ascites to establish the subcutaneous transplantation tumor model. Drug was administrated when the tumor volume reached 0.06cm3. The experimental grouping and intervention measures are as follows（n=6）： mice in control group received no intervention, and fed normally. Mice in experiment group were treated with respiratory tract inhalation of Chinese medicine gas, daily administration 6h, once a day, continuous administration of 21 days. Mice in adriamycin（ADM） group were intraperitoneally injected 1mg/kg of ADM, once a week, a total of 3 times. General signs of the mice including activity and eating status were observed daily. The body weight of mice was measured every other day. Blood samples were taken 2 days after stopping drug inhalation by inner canthus vein bleeding for detection of CD3~＋, CD4~＋ and CD8~＋ T lymphocyte ratio by flow cytometry. The subcutaneous nodule was dissected and weighed, and some tumor tissues were pathologically observed. Part of tumor tissues was prepared into single cell suspension to detect the cell apoptosis by flow cytometry. Results The CD3~＋, CD4~＋ T lymphocytes ratio and the ratio CD4~＋/CD8~＋ were significantly higher in experiment group than in control group（P〈0.05）. The weight of subcutaneous transplantation tumor was significantly lower in experiment group and ADM group than in control group（P〈0.01）, but no significant difference existed between experiment group and ADM group（P〈0.05）. The apoptotic rate increased significantly in experiment group than in control group（P〈0.05）. Histopathological observation showed that, in control group, the transplanted tumor tissue had rich cells arranged clo

关 键 词：投药 吸入 膏剂 免疫系统 肉瘤180 细胞凋亡 

分 类 号：R73-354[医药卫生—肿瘤]