羟乙基淀粉对大鼠心肌缺血再灌注后无复流的影响  

Effects of HES 130/0.4 on no-reflow after myocardial ischemia-reperfusion injury in rats

作　　者：黄崇安[1] 何丽娜[1] 孙嘉利[1] 应安娜[1] 叶永挺 蔡琪[1] 袁琳波[1] 花春艳[1] 

机构地区：[1]温州医科大学生理学教研室,浙江温州325035

出　　处：《中国病理生理杂志》2016年第3期411-417,共7页Chinese Journal of Pathophysiology

基　　金：浙江省教育厅资助项目(No.Y201223758);温州市科技计划项目(No.Y20140230);温州医科大学学生新苗课题(No.wyx201401024;No.wyx2015101072)

摘　　要：目的:探讨羟乙基淀粉(HES 130/0.4)对大鼠心肌缺血再灌注中无复流现象的影响及机制。方法:36只SD大鼠随机分为缺血-再灌注组(IR组),生理盐水组(NS-IR组),羟乙基淀粉组(HES-IR组)和假手术组(sham组)。NS-IR和HES-IR组在缺血30 min时分别静注生理盐水和HES 130/0.4,再灌注180 min后以Evans blue、Thilflavin S和TTC染色测定心肌梗死面积与无复流范围,同时检测心肌肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白I(c Tn I)含量和髓过氧化物酶(MPO)活性。培养心肌微血管内皮细胞,随机分为正常对照组(Con组),缺氧-复氧组(H/R组),生理盐水组(NS-H/R组)和羟乙基淀粉组(HES-H/R组),以缺氧复氧法模拟急性缺血再灌注,测定游离钙离子浓度、细胞活力和凋亡率。结果:HES-IR组大鼠心肌梗死面积、无复流范围、心肌酶CK-MB、c Tn I和MPO与IR组相比均减少(P<0.05)。HES-H/R组细胞内游离钙离子浓度和细胞凋亡率较H/R组亦明显减少(P<0.05),而细胞活力增高(P<0.05)。结论:HES 130/0.4能改善心肌缺血-再灌注后的无复流现象,其机制不仅涉及对中性粒细胞激活、浸润的抑制,还与减轻内皮细胞钙超载有关。AIM：To observe the effects and mechanisms of hydroxyethylstarch（ HES） 130/0.4 on no-reflow phenomenon after myocardial ischemia-reperfusion in rats.METHODS：SD rats were randomly divided into 4 groups：sham operation group,ischemia-reperfusion（ IR,treated with normal saline） group,normal saline ischemia-reperfusion（ NS-IR,treated with NS） group and HES ischemia-reperfusion（ HES-IR,treated with HES） group.Myocardial infarct size and no-reflow range were determined by staining methods,and the activities of myocardial enzymes（ CK-MB,c Tn I and MPO） were measured.Meanwhile,cardiac microvascular endothelial cells of the rat were cultured and divided into 4groups：control group,hypoxia/reoxygenation（ H/R） group,NS-H/R group and HES-H/R group.Acute ischemia reperfusion models were simulated,and the concentration of calcium ions was measured.The relative cell activity was evaluated by CCK-8 assay,and the apoptotic rate was detected by flow cytometry.RESULTS：In HES-IR group,the myocardial infarct size,the no-reflow zone,CK-MB,c Tn I and MPO activity were all significantly lower than those in IR group（ P〈 0.05）.In microvascular endothelial cells,the concentration of calcium ions and the apoptotic rate in HES-H/R group were significantly decreased,while the relative cell activity increased compared with H/R group（ P〈 0.05）.CONCLUSION：HES reduces no-reflow in acute myocardial ischemia-reperfusion.The mechanism may be involved in the inhibition of both the infiltration of neutrophils and the calcium overload of endothelial cells.

关 键 词：羟乙基淀粉 心肌再灌注损伤 无复流 髓过氧化物酶 钙离子 

分 类 号：R363.2[医药卫生—病理学] R541.4[医药卫生—基础医学]