产雌马酚梭菌C1对小鸡沙门氏菌攻毒保护效果  

Protective effect of an equol-producing Clostridium C1 against Salmonella infection in chicken

作　　者：尹业师[1] 方丹[2] 朱立颖[1] 刘伟[1] 吴健[2] 王欣[1] 

机构地区：[1]浙江省农业科学院植物保护与微生物研究所/农产品质量安全国家重点实验室培育基地,浙江杭州310021 [2]华中农业大学动物医学院,湖北武汉430070

出　　处：《浙江农业学报》2016年第2期234-239,共6页Acta Agriculturae Zhejiangensis

基　　金：国家自然基金(3100097);"十二五"农村领域国家科技计划(2013BAD10B02-03)

摘　　要：部分梭菌对机体的免疫系统具有明显的调节作用,可开发成新型益生菌。由于鸡盲肠内容物中梭菌含量较高,从小鸡肓肠内容物中分离培养功能性梭状芽孢杆菌,并在小鸡中初步研究其对沙门氏菌攻毒的保护效果。该研究首先采用梭菌选择性培养基,从小鸡肓肠内容物中分离获得了一株可以将大豆素转化为雌马酚的革兰氏阳性纤维状梭菌C1,以四环素为对照,研究梭菌C1对小鸡沙门氏菌攻毒的保护作用。结果表明,梭菌C1组和四环素组均具有降低肓肠内容物中沙门氏菌活菌数和促进小鸡生长的功能,推测梭菌C1具有开发为四环素替代品的潜能。虽然较高浓度的S-雌马酚(5 mg·m L-1)在体外对沙门氏菌有一定的抑制作用,梭菌C1也具有将大豆素转化为雌马酚的能力,但梭菌C1在体外对沙门氏菌的抑制作用并不明显,其益生机制还有待进一步研究。Some Clostidium could regulate the development of the host immune system. Hence,these Clostidium strains had the potential to be used as probiotics. Since the population of Clostridium in chicken cecal contents was relative high,the aims of this study were to isolate functional Clostidium strains from chicken cecal contents and subsequently study their protective effect against Salmonella infection. In this study,a Gram-positive filamentous Clostridium sp C1 which could transfer daidzein to equol,was isolated from chicken cecal contents and then the animal experiments were conducted to study the protective effect of Clostridium sp C1 on Salmonella infection. As the tetracycline treated group did,the Clostridium sp C1 also had effect on decreasing the number of Salmonella in cecal contents and promoting the growth of chicken. Thus,the Clostridium sp C1 had the potential to be used as a tetracycline substitute. Although,the high concentration of S-equol（ 5 mg·m L^- 1） had the function on inhibiting the growth of Salmonella in vitro and the Clostridium sp C1 had the function of transfering daidzein to S-equol,the inhibition effects of Clostridium sp C1 on Samonella growth was not observed in vitro. The probiotic mechanism of Clostridium sp C1 need to be further studied.

关 键 词：梭菌 雌马酚 小鸡 沙门氏菌 

分 类 号：S831.7[农业科学—畜牧学]