针刺结合丹红注射液对大鼠脑缺血再灌注损伤的保护作用  

Neuroprotective effect of acupuncture combined with Danhong injection on focal cerebral ischemia / reperfusion injury in rats

作　　者：曾兆禄[1] 韩继超[2] 薛云 马玉奎 

机构地区：[1]山东省临朐县人民医院,临朐山东262600 [2]济南市第一人民医院,济南山东250011 [3]山东省药学科学院,济南山东250033

出　　处：《中国比较医学杂志》2016年第2期62-66,共5页Chinese Journal of Comparative Medicine

摘　　要：目的研究针刺结合丹红注射液对大鼠脑缺血再灌注损伤有无保护作用。方法采用大脑中动脉内栓线阻断法(MCAO)制备大鼠大脑中动脉脑缺血再灌注损伤模型,缺血2 h后将线拔出实现再灌注,分别于再灌注后立刻静脉注射丹红注射液2 m L/kg,并针刺百会、足三里,每天1次,连续3 d,72 h后采用Zea-Longa的5级4分制评分方法进行神经功能评分,TTC染色法测定脑梗死面积,Western blot法检测缺血侧脑皮层中Bcl-2与Bax的蛋白表达。结果与模型和单用丹红注射液组比较,针刺结合丹红注射液治疗后大鼠神经功能缺陷评分明显降低,脑梗死面积减少,Bcl-2的蛋白表达增多,Bax蛋白表达减少。结论针刺结合丹红注射液比单用丹红注射液对大鼠脑缺血再灌注损伤具有更明显的保护作用。Objective To investigate the neuroprotective effect of acupuncture combined with a Chinese medicine, Danhong injection, on focal cerebral ischcmia/reperfusion injury in rats. Methods Middle cerebral artery （MCA） occlusion was prepared by putting a nylon suture in the MCA. The MCA blood flow in the animals was restored at two hours after the MCA occlusion by withdrawing the suture to the external carotid artery （ECA）. The acupoints ＂Baihui＂ （DU20） and ＂Zusanli＂ （ST36） were acupunctured and stimulated by twirling the acupuncture needle after reperfusion, and the rats were also administered with Danhong injection by intravenous injection, once every day for three days. Zea-Longa scores were recorded to assess the changes of neurological function. The rats were sacrificed at 72 h after reperfusion. Triphenyhetrazolium chloride （TTC） staining was used to measure cerebral infarction size. Western blot was used to test the level of Bcl-2 and Bax in the ischemic penumbra. Results The acupuncture combined with Danhong injection treatment group displayed markedly lower neurologic deficit scores, reduced ischemic infarct surface, increased expression of Bcl-2 protein, and reduced expression of Bax protein, compared with those in the model group and Danhong injection alone group. Conclusions Acupuncture combined with Danhong injection show more significant neuroprotective effect than Danhong injection treatment alone on rat models of cerebral ischemia/reperfusion injury.

关 键 词：针刺 脑缺血再灌注损伤 BCL-2 BAX 

分 类 号：R-33[医药卫生]