高频电刺激卒中后抑郁大鼠小脑顶核对下丘脑外侧区神经元放电及氨基酸类递质的影响  

Effect of High-Frequency Stimulation in the Medial Cerebellar Nucleus on Neuronal Activity and Neurotransmitters in the Lateral Hypothalamic Area of Rats

作　　者：李岩[1] 隋汝波[1] 李媛[1] 张敏[2] 张欣[3] 

机构地区：[1]辽宁医学院附属第一医院神经内科,辽宁锦州121000 [2]辽宁医学院附属第一医院普外科,辽宁锦州121000 [3]抚顺矿务局总医院药剂科,辽宁抚顺113008

出　　处：《中山大学学报（医学科学版）》2016年第1期34-39,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(81241050;81371461)

摘　　要：【目的】通过观察高频电刺激卒中后抑郁(PSD)模型大鼠小脑顶核(MCN)对下丘脑外侧区(LHA)神经元放电及氨基酸类递质的影响,来探讨电刺激MCN治疗PSD的机制。【方法】将60只SD雄性大鼠随机分为对照组20只,缺血性卒中组20只,PSD模型组20只。应用行为学测试评定抑郁的行为学改变;应用细胞外单位记录观察高频电刺激MCN对LHA神经元放电的影响;脑内微透析收集LHA脑脊液,结合高效液相色谱(HPLC)检测氨基酸类递质的变化。【结果】高频电刺激MCN,对照组、缺血性卒中组及PSD模型组大鼠LHA神经元的反应均以抑制性为主,且平均放电频率均较刺激前明显下降(P<0.01);高频电刺激MCN,LHA内谷氨酸(Glu)和γ-氨基丁酸(GABA)水平较刺激前明显增加(P<0.01)。【结论】高频电刺激MCN可抑制LHA神经元的电活动,增加LHA内Glu和GABA的水平,其抑制LHA神经元的效应可能是通过小脑-下丘脑直接纤维投射实现。【Objective】 To study the effect of high frequency stimulation（HFS） in Medial cerebellar nucleus（MCN） on the neuronal activities of lateral hypothalamic area（LHA） in post-stroke depression（PSD） rats, and the mechanisms there of. 【Methods】60 Sprague-Dawley rats were divided into three groups ： control group（n = 20）, stroke group（n = 20） and PSD group（n = 20）.Electrophysiological recordings were made in anaesthetized rats to investigate the effects of HFS-MCN on the firing rate of the LHA neurons. Brain microdialysis combined with high-performance liquid chromatography（HPLC） was applied to detect glutamate（Glu）and γ-aminobutyric acid（GABA） levels in LHA. 【Results】 HFS-MCN caused an inhibitory reaction of the majority of neurons recorded in the LHA in control group, stroke group and PSD model group. The mean firing rate of LHA inhibited neurons was significantly decreased（P〈0.01）. The levels of Glu and GABA in the LHA were increased（P〈0.01）. 【Conclusion】 HFS-MCN restrained the electrical activity of LHA neurons and increased the level of Glu and GABA. These inhibitory effects were probably realized by the cerebellum- hypothalamic pathways.

关 键 词：卒中后抑郁 高频电刺激 小脑顶核 下丘脑外侧区 谷氨酸 γ-氨基丁酸 

分 类 号：R741.05[医药卫生—神经病学与精神病学]