黑果枸杞及其伪品白刺果的HPLC指纹图谱研究  

Study on the HPLC Fingerprint of Lycium ruthenicum and Its Adulterant Nitraria tangutorum

作　　者：娄涛涛 陀扬凌 金玲[1] 陈天强[1] 张旭[1] 

机构地区：[1]成都中医药大学药学院/教育部中药材标准化重点实验室/中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都610075

出　　处：《中国药房》2016年第9期1245-1249,共5页China Pharmacy

基　　金：四川省教育厅重点项目(No.10ZA090)

摘　　要：目的:建立黑果枸杞及其伪品白刺果的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Diamonsil ODS,流动相为乙腈-水(梯度洗脱),流速为0.8 ml/min,检测波长为270 nm,柱温为30℃,进样量为10μl。以芦丁为参照物,对13批黑果枸杞与4批白刺果采用"中药色谱指纹图谱相似度评价系统"软件进行相似度、主成分和聚类分析。结果:13批黑果枸杞有20个共有峰,其中12批黑果枸杞的相似度均>0.9,明显高于4批白刺果的相似度。13批黑果枸杞密集分布在一个区域,而4批白刺果分布在此区域外。13批黑果枸杞聚为一类,4批白刺果聚为一类;进一步运用聚类分析对13批黑果枸杞进行质量评价,总共可聚为4类。结论:所建立的指纹图谱可为黑果枸杞的鉴别和质量评价提供参考。OBJECTIVE：To establish the HPLC fingerprint of Lycium ruthenicum and its falsity Nitraria tangutorum. METHODS：HPLC was performed on the column of Diamonsil ODS with mobile phase of acetonitrile-water（gradient elution）at a flow rate of 0.8 ml/min,detection wavelength was 270 nm,column temperature was 30 ℃,and the injection volume was 10 μl. With the references of rutin,TCM chromatographic fingerprint similarity evaluation system was used for the similarity,principal component and cluster analysis of 13 batches of L. ruthenicum and 4 batches of N. tangutorum. RESULTS：There were totally 20 common peaks in 13 batches of L.ruthenicum,similarity of the 12 batches L. ruthenicum of higher than 0.9,which was significantly higher than the similarity of 4 batches of adulterants. 13 batches of L. ruthenicum was in a densely populated area,and the 4 batches of adulterants distributed outside this region. 13 batches of L. ruthenicum clustered together,and 4 batches of adulterants into one group;further cluster analysis was used for the quality evaluation of 13 batches of L. ruthenicum,total clustered into four categories. CONCLUSIONS：The established fingerprint can provide reference for identification and quality evaluation of L. ruthenicum.

关 键 词：黑果枸杞 白刺果 高效液相色谱法 指纹图谱 化学计量学 质量控制 

分 类 号：R927.1[医药卫生—药学]