ALC1介导的MAPK信号通路激活对肝癌细胞增殖的影响  

ALC1 promote the proliferation of hepatocellular carcinoma cells via the activating of MAPK pathway

作　　者：李杨[1] 冯丽[1] 陈娟[1] 成炜[1] 刘珊珊[1] 刘玉荣[1] 马宁芳[1] 

机构地区：[1]广州医科大学基础学院组织学与胚胎学教研室,广东广州510182

出　　处：《解剖学研究》2016年第1期48-51,共4页Anatomy Research

基　　金：广州市教育局"羊城学者"科研项目(12A016G);国家自然科学基金(30872508)

摘　　要：目的探讨ALC1促肝癌细胞增殖作用与MAPK信号通路的关系。方法 qPCR检测肝癌细胞系ALC1 mRNA表达;MTS实验及平板克隆实验验证ALC1 siRNA干扰后对Huh7细胞增殖能力的影响;Western blot法从蛋白水平检测验证ALC1 siRNA干扰后MEK/ERK通路相关蛋白的磷酸化改变。结果肝癌细胞株Huh7、HepG2及BEL-7402均有ALC1 mRNA表达,其中Huh7细胞ALC1 mRNA表达水平明显高于其它细胞株。MTS实验结果显示ALC1 siRNA干扰后Huh7细胞增殖速度与对照组相比明显下降(P<0.05);平板克隆实验结果显示siRNA干扰ALC1表达后Huh7细胞克隆体积变小,克隆形成数量与对照组相比明显减少,差异有显著性(P<0.05)Western blot实验结果显示与对照组相比ALC1siRNA干扰可明显降低Huh7细胞MEK、ERK蛋白的磷酸化水平(P<0.05)。结论 ALC1高表达于肝癌Huh7细胞;ALC1介导了MAPK信号通路激活对Huh7细胞增殖能力的影响。Objective To explore the correlation between the promote effects of ALC1 on the proliferation of liver cancer cells and MAPK Pathway activity.Methods The expression of ALC1 mRNA expression were detected with q RT-PCR in hepatocellular carcinoma cell line. The the colony formation and MTS experiment were applied to test the effects of proliferation after ALC1 RNA interference and Western blot was uesd to detected the phosphorylation of MEK and ERK. Results ALC1 were expressed in Huh7,HepG2 and BEL-7402 cells. The higher expression level of ALC1 was found in Huh7 cells when compared with that of HepG2 and BEL-7402 cells. The results of MTS experiment showed that the multiplication rate of Huh7 cell were significantly decreased than the of control group after treating with ALC1 siRNA（P〈0.05）,the colony formation rate of Huh7 cells also decreased significantly following siRNA treatment（P〈0.05）. Western blot results showed that the total MEK and ERK protein levels were not changed obviously after ALC1 siRNA treatment,but the p-MEK and p-ERK protein levels were remarkably decreased（P〈0.05）following the expression of ALC1 was restrained. Conclusion The high expression level of ALC1 was significantly found in Huh7 cells.ALC1 promote the proliferation of hepatocellular carcinoma cells by activating the MAPK Pathway.

关 键 词：ALC1 肝癌细胞 MAPK 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]