结肠癌细胞上皮间质转化对β1整合素表达及裸鼠体内肝转移的影响  

Influence of epithelial - mesenchymal transition of human colon carcinoma on integrin -β1 expres- sion and hepatic metastasis in vivo

作　　者：郑淑龙 高军[1] 高品[1] 纪奕顺 吕春龙[1] 张敏[1] 

机构地区：[1]青岛大学附属医院普外科,266003

出　　处：《中华实验外科杂志》2016年第3期571-573,共3页Chinese Journal of Experimental Surgery

基　　金：山东省自然科学基金（Y2007C030）

摘　　要：目的观察结肠癌SW480细胞发生上皮．间充质转化（EMT）对β1整合素表达及裸鼠体内肝转移的影响。方法用10μg/L转化生长因子-β1（TGF-β1）诱导72h后检测SW480细胞发生EMT。实时定量聚合酶链反应（Real-timePCR）检测EMT前后SW480细胞β1整合素mRNA表达。裸鼠开腹于脾脏被膜下注射SW480细胞建立体内肝转移模型，30d后处死裸鼠，计算肝转移癌的数量、大小及重量。结果EMT后SW480细胞β1整合素mRNA表达较EMT前明显增强（t=3．764，P〈0．05）；EMT后结肠癌细胞裸鼠体内肝转移的数量为（9．92±1．44）个／只、体积为（33．82±0．33）mm^3／只、重量为（0．2315±0．0046）g／只，均较EMT前[肝转移的数量为（4．44±0．96）个／只、体积为（16．64±O．61）mm^3／只、重量为（0．1183±0．0037）g/只]明显增高（P〈0．05）。结论结肠癌细胞发生EMT后β1整合素表达升高，体内肝转移能力增强。Objective To explore the effect of epithelial - mesenchymal transition （EMT） of hu- man colon carcinoma cell line SW480 on integrin -β1 expression and hepatic metastasis in nude mice. Methods EMT of SW480 ceils was induced by transforming growth factor - β1 （TGF - 131, 10 μg/L） for 72 h. The expression of integrin - β1 mRNA in SW480 cells was detected by real - time quantitative poly- merase chain reaction （Real -time PCR）. The models of hepatic metastasis in nude mice were established by the intrasplenic injection of SW480 cells. Thirty days later, the number, size and weight of hepatic metas- tases in the nude mice were measured. Results As compared with that before EMT, the mRNA expression of integrin- β1 in SW480 cells after EMT was markedly increased （t =3. 764,P 〈0. 05）. In vivo, the number （9. 92 ± 1.44）/mouse, volume （33. 82 ±0. 33） mm^3/mouse, and weight （0. 231 5 ±0. 004 6） g/mouse of hepatic metastases in the nude mice after EMT were significantly increased （P 〈 0. 05）. Conclusion The expression of integrin - β1 and hepatic metastasis ability of human colon carcinoma cells in vivo can be ele- vated after EMT.

关 键 词：结肠癌 上皮-间充质转化 Β1整合素 肝转移 

分 类 号：R735.34[医药卫生—肿瘤]