慢传输便秘患者结肠组织差异蛋白质组学研究  

Differential proteomics analysis of colonic tissues in patients with slow transit constipation

作　　者：刘韦成[1] 万松林[1] 张秋雷[1] 张延涛[1] 任相海[1] 丁召[1] 郑科炎[1] 秦前波[1] 李颀[1] 吴云华[1] 江从庆[1] 钱群[1] 

机构地区：[1]武汉大学中南医院结直肠肛门外科湖北省肠病医学临床研究中心肠病湖北省重点实验室湖北省卫生和计划生育委员会结直肠肛门外科质量控制中心湖北省医学会结直肠肛门外科学分会,430071

出　　处：《中华实验外科杂志》2016年第3期607-610,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金（81500505、81570492）;武汉市中青年医学骨干人才项目;湖北省科技计划项目（2015CFB636、2011CDB521）;武汉大学自主科研项目（303410500160）;武汉大学中南医院科研基金项目（22#）

摘　　要：目的观察慢传输型便秘型（STC）患者与正常人结肠组织蛋白质表达的差异。方法采用双向凝胶电泳（2-DE）技术，计算机辅助的图像分析以及质谱鉴定方法，对5例STC患者与5例正常人结肠组织蛋白质进行分离和比较分析。结果正常对照组平均胶蛋白斑点数为239个，STC组平均胶蛋白斑点数为215个，与正常对照组平均胶匹配点数为197个，匹配率为82％，有14个蛋白质点的表达量差异有统计学意义（差异倍数〉10，P〈0．05），其中有12个蛋白点表达发生明显上调，有2个蛋白点表达发生明显下调。结论STC患者与正常人结肠组织蛋白质表达谱差异有统计学意义，这些差异蛋白可能参与了STC的发病机制．Objective To investigate and screen the different expression patterns of proteins be- tween slow transit constipation （STC） group and normal group using a comparative proteomic approach. Methods Two - dimensional eleetrophoresis was applied to separate the proteins in specimens from 5 STC patients and 5 normal controls. The proteins with statistically significant differential expression between two groups were identified by using computer aided image analysis and matrix assisted laser desorption ioniza- tion tandem time of flight mass spectrometry （MALDI- TOF- MS）. Results A total of 239 protein spots were identified in the average gel of the normal controls, and 215 in patients with STC. A total of 197 pro- tein spots were matched and the mean matching rate was 82%. There were 14 protein spots which were ex- pressed with statistically significant differences. Of those 14 protein spots, the expression of 12 spots was increased markedly, while 2 were decreased significantly. Conclusion The proteomics expression in co- lonic specimens of STC patients is statistically significantly different from that of normal contrils, which may be associated with the pathogenesis of STC.

关 键 词：蛋白质组学 双向凝胶电泳 慢传输型便秘 

分 类 号：R730.43[医药卫生—肿瘤]