构建聚己内酯／明胶电纺复合支架修复颌骨缺损的研究  

Preparation of the compound electrospunpoly （ e - caprolactone ）/gelatin fibrous scaffolds： a study of repairing mandible defects

作　　者：花蕾[1] 卜寿山[1] 曹晓建[1] 张继生[1] 庄海[1] 尹林[1] 

机构地区：[1]南京医科大学第一附属医院口腔科,210029

出　　处：《中华实验外科杂志》2016年第3期730-733,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：江苏省自然科学基金（BK2011844）;江苏省“六大人才高峰”第八批高层次人才项目（WSW-021）;南京医科大学第一附属医院创新团队工程（IRT-015）;江苏省卫计委科研项目（H201504）

摘　　要：目的观察自主构建的聚已内酯／明胶电纺复合支架生物相容性及对颌骨缺损修复的作用。方法运用电纺技术和明胶-甲基丙烯酰胺水凝胶制备聚已内酯／明胶复合支架。通过细胞计数试剂盒（CCK-8）检测小鼠前成骨细胞与支架共培养的细胞活性。并在新西兰大白兔颌骨缺损模型中植入电纺复合支架。术后12周对新生骨组织进行微型计算机断层扫描（Micro—CT）扫描，苏木素．伊红（HE）染色与马松（Masson）染色。结果CCK-8结果显示，在7d时实验组（1．9393±0．4174）与对照组（2．4574±0．3180）的细胞活性差异无统计学意义（P〉0．05）。通过Micro—CT检测，实验组新生骨密度（0．220±0．253）与新生组织构成比（11．941±3．046）均高于对照组（0．179±0．348、5．809±2．433，P〈0．05）。组织学研究结果显示，在实验组中未见纤维结缔组织侵入再生区域，复合支架部分降解，支架内层可见片层状新生骨组织。结论这种聚已内酯／明胶电纺复合支架，具有良好的生物相容性，能有效地维持骨再生空间，促进新骨形成。Objective To study the cell compatibility of the compound electrospun poly （ e - cap- rolactone）/gelatin fibrous scaffolds for mandibular defect reconstruction. Methods The compound scaf- folds of poly （e- caprolactone）/gelatin electrospun membranes and gelatin- methacrylamide hydrogel （GelMA） were prepared. Cell counting kit -8 （CCK -8 ） was used to analyze the cell compatibility of MC3T3 - E1 cultured with the scaffold. Then we planted the scaffold into animal model of bone defect of the mandibular body in 6 New Zealand white rabbits. The new bone tissue was subjected to microcomputed tomographic （micro- CT） examination and stained with hematoxylin- eosin （HE） and Masson at 12th week postoperatively. Results The result of CCK - 8 assay showed that there was no significance in cell viability between experimental group （ 1. 939 3 ±0.417 4） and control group （2. 457 4 ±0. 318 0）. The experimental group showed higher new bone mineral density （BMD, 0. 220 ± 0. 253 ） and bone volume/tis- sue volume （BV/TV, 11. 941 ±3.046） than the control group （BMD, 0. 179 ±0. 348; BV/TV, 5. 809 ± 2. 433, P 〈 0. 05 ） by micro - CT examination. Histological study indicated that there was no fiber connec- tive tissue invading into bone defect areas in experimental group, scaffolds had been partly degraded and newly generated bone with lamellar structure was found in defect areas. Conclusion The compound elec- trospun gelatin/poly （e -caprolactone ） fibrous scaffolds have a good biocompatibility, can effectively maintain space for bone regeneration and promote new bone formation.

关 键 词：电纺技术 复合支架 骨缺损重建 

分 类 号：R782.405[医药卫生—口腔医学]