冠状动脉搭桥术后静脉桥再狭窄动物模型的建立  

Establishment of Animal Model of Vein Graft Restenosis After Coronary Artery Bypass Grafting

作　　者：胡佳心[1] 王侃[1] 阮新民[1] 

机构地区：[1]广州中医药大学附属第二医院心血管二科,广东省广州市510105

出　　处：《中国动脉硬化杂志》2016年第2期198-202,共5页Chinese Journal of Arteriosclerosis

基　　金：广东省科技计划项目(20140212)

摘　　要：目的建立冠心病冠状动脉搭桥术后移植静脉桥再狭窄的动物模型。方法 26只新西兰兔随机分成3组:高脂移植组(n=10)、单纯移植组(n=10)、正常对照组(n=6),分别给予自体颈外静脉移植加高脂饲料、自体颈外静脉移植加普通饲料、普通饲料喂养。移植组均进行颈外静脉反向吻合于颈动脉的搭桥手术,术后用彩色超声检测血流量,证明静脉桥通畅。2个月后,用多普勒检测静脉桥血流量及内膜厚度,取组织标本作病理切片检查,以了解静脉桥有无血栓,内皮细胞及平滑肌细胞有无增生迁移及血管基质有无增生等。结果 (1)术毕所有静脉桥均通畅。(2)术后2个月彩色超声检查示高脂移植组与单纯移植组和正常对照组相比,静脉桥内膜有斑块形成,狭窄明显,内膜明显增厚。(3)术后2个月病理切片显示高脂移植组的静脉桥内皮细胞脱落、内膜增生、平滑肌细胞移行增殖和脂质沉积等典型的粥样硬化变化,而单纯移植组和正常对照组则不明显。结论兔颈外静脉反向吻合于颈动脉的静脉搭桥动物模型是可靠的,其为研究静脉桥术后再狭窄提供了既简单又较理想的模型。Aim To establish the animal model of vein graft restenosis after coronary artery bypass grafting in patients with coronary artery disease. Methods 26 New Zealand rabbits were randomly divided into three groups：（ 1）high fat graft group（ n = 10） ： rabbits treated by autologous external jugular vein grafting to common carotid artery and fed by high fat diet;（ 2） pure graft group（ n = 10） ： rabbits treated by autologous external jugular vein grafting to common carotid artery and fed by normal diet;（ 3） normal control group（ n = 6） ： rabbits fed by normal diet. The graft groups were performed by the external jugular vein anastomosis to the carotid artery bypass surgery. Blood flow was detected by color Doppler ultrasound,and the patency of vein graft was proved. Two months later,Doppler was used to detect the blood flow and intima thickness of vein graft,and the tissue specimens were taken for pathological examination,in order to understand whether there is thrombus in vein graft,proliferation and transfer of endothelial cell and smooth muscle cell,and proliferation of vascular matrix,etc. Results（ 1） All vein grafts were unobstructed after operation.（ 2） Two months after operation,color Doppler ultrasound examination showed that there were plaque formation,obvious stenosis and intima thickening of the vein grafts in high fat graft group,compared with the pure graft group and the normal control group.（ 3）The pathological examination showed that there were typical atherosclerotic changes such as shedding of the endothelial cells,intimal hyperplasia,smooth muscle cell migration,proliferation and lipid deposition on vein grafts in high fat graft group,while the changes in pure graft group and normal control group were not obvious. Conclusions The animal model of rabbit external jugular vein anastomosis to the carotid artery is reliable. It provides a simple and ideal model for the study of restenosis after vein grafts.

关 键 词：冠状动脉搭桥术 静脉桥 再狭窄 颈外静脉 颈动脉 动物模型 

分 类 号：R-332[医药卫生]