Effects of microtubule-associated protein tau expression on neural stem cell migration after spinal cord injury  

作　　者：Zhi-ping Qi Guo-xiang Wang Peng Xia Ting-ting Hou Hong-li Zhou Tie-jun Wang Xiao-yu Yang 

机构地区：[1]Department of Orthopedics, the Second Hospital of lilin University, Changchun, Jilin Province, China [2]Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China

出　　处：《Neural Regeneration Research》2016年第2期332-337,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81250016,31572217

摘　　要：Our preliminary proteomics analysis suggested that expression of microtubule-associated protein tau is elevated in the spinal cord after injury. Therefore, the first aim of the present study was to examine tau expression in the injured spinal cord. The second aim was to determine whether tau can regulate neural stem cell migration, a critical factor in the successful treatment of spinal cord injury. We established rat models of spinal cord injury and injected them with mouse hippocampal neural stem cells through the tail vein. We used immunohistochemistry to show that the expression of tau protein and the number of migrated neural stem cells were markedly increased in the injured spinal cord. Furthermore, using a Transwell assay, we showed that neural stem cell migration was not affected by an elevated tau concentration in the outer chamber, but it was decreased by changes in intracellular tau phosphorylation state. These results demonstrate that neural stem cells have targeted migration capability at the site of injury, and that although tau is not a chemokine for targeted migration of neural stem cells, intracellular tau phosphorylation/dephosphorylation can inhibit cell migration.Our preliminary proteomics analysis suggested that expression of microtubule-associated protein tau is elevated in the spinal cord after injury. Therefore, the first aim of the present study was to examine tau expression in the injured spinal cord. The second aim was to determine whether tau can regulate neural stem cell migration, a critical factor in the successful treatment of spinal cord injury. We established rat models of spinal cord injury and injected them with mouse hippocampal neural stem cells through the tail vein. We used immunohistochemistry to show that the expression of tau protein and the number of migrated neural stem cells were markedly increased in the injured spinal cord. Furthermore, using a Transwell assay, we showed that neural stem cell migration was not affected by an elevated tau concentration in the outer chamber, but it was decreased by changes in intracellular tau phosphorylation state. These results demonstrate that neural stem cells have targeted migration capability at the site of injury, and that although tau is not a chemokine for targeted migration of neural stem cells, intracellular tau phosphorylation/dephosphorylation can inhibit cell migration.

关 键 词：nerve regeneration spinal cord injury tau protein neural stem cells transwelI chambers phosphatase 2A cell transplantation PHOSPHORYLATION MIGRATION okadaic acid C2-ceramide neural regeneration 

分 类 号：R651.2[医药卫生—外科学]