二甲基亚砜诱导骨髓间充质干细胞向心肌细胞的分化  

Dimethyl sulfoxide induces differentiation of bone marrow mesenchymal stem cells into myocardial cells

作　　者：孙龙云[1] 

机构地区：[1]张家口学院,河北省张家口市075000

出　　处：《中国组织工程研究》2016年第1期26-30,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:大量研究证明,骨髓间充质干细胞在一定的诱导条件下可定向分化为心肌细胞。二甲基亚砜是目前常用的诱导分化剂之一,其作用机制主要是通过抑制c-myc基因表达,从而降低细胞内源性聚腺苷二磷酸核苷水平。目的:探讨二甲基亚砜诱导骨髓间充质干细胞定向分化为心肌细胞的可行性及最佳诱导浓度。方法:通过体外分离培养SD大鼠骨髓间充质干细胞,并用二甲基亚砜诱导使其定向分化为心肌细胞,依诱导剂二甲基亚砜的浓度分为3组,浓度分别为0.6%、0.8%和1.0%,不加诱导剂为空白对照组。诱导72 h后,去除诱导培养基,加入不含诱导剂的普通培养基继续培养4周。结果与结论:形态学及免疫细胞化学检测显示二甲基亚砜可以在体外诱导骨髓间充质干细胞定向分化为心肌样细胞并表达心肌特异性蛋白desmin、α-actin、cT nT、cT n I和P38MAPK,且1.0%二甲基亚砜是最佳诱导浓度。免疫荧光双标染色及电镜结果均进一步证实二甲基亚砜可以诱导骨髓间充质干细胞获得心肌分化表型。BACKGROUND： Numerous studies have demonstrated that bone marrow mesenchymal stem cells can be induced to differentiate into myocardial cells under certain conditions. Dimethyl sulfoxide is one of the commonly used inducers, and its mechanism is mainly by inhibiting the c-myc gene expression, thus reducing endogenous poly（adenosine diphosphate nucleotide） level.OBJECTIVE： To study the feasibility of dimethyl sulfoxide inducing the myocardial differentiation of bone marrow mesenchymal stem cells and its optimal concentration. METHODS： Bone marrow mesenchymal stem cells from Sprague-Dawley rats were isolated and cultured in vitro, and then induced by dimethyl sulfoxide to differentiate into myocardial cells. According to the concentrations ofdimethyl sulfoxide, there were three groups： 0.6%, 0.8% and 1.0% group. Additionally, a blank control group with no induction was set up. After 72 hours of induction, induction media were removed, and cells were then cultured in normal media for 4 weeks. RESULTS AND CONCLUSION： Morphology and immunocytochemistry detection results confirmed that dimethyl sulfoxide could induce the differentiation of bone marrow mesenchymal stem cells into myocardial cells in vitro, and differentiated cells expressed desmin, α-actin, cT nT, cT nI and P38 MAPK. The optimal induced concentration of dimethyl sulfoxide was 1.0%. Immunofluorescence double staining and electron microscope results further confirmed that dimethyl sulfoxide could induce the myocardial differentiation of bone marrow mesenchymal stem cells.

关 键 词：二甲亚砜 骨髓 间质干细胞 肌细胞 心脏 细胞分化 组织工程 干细胞 骨髓干细胞 二甲基亚砜 骨髓间充质干细胞 心肌细胞 诱导分化 

分 类 号：R394.2[医药卫生—医学遗传学]