隐丹参酮对肾癌干细胞增殖和凋亡的影响  

Cryptotanshinone effects on the proliferation and apoptosis of renal carcinoma stem cells

作　　者：冯敏 贾明华 

机构地区：[1]郑州颐和医院肾内科,河南省郑州市450008

出　　处：《中国组织工程研究》2016年第1期49-54,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:前期研究发现隐丹参酮对多种肿瘤具有抑制作用,但是对肾癌的影响尚未有报道。目的:探讨隐丹参酮对肾癌干细胞增殖和凋亡的影响。方法:采用免疫磁珠法分离纯化CD133^+肾癌干细胞。通过MTT法检测不同质量浓度(0,0.2,1.5 mg/L)隐丹参酮对CD133^+肾癌干细胞增殖的影响;流式细胞仪检测不同质量浓度(0,0.2,1.5 mg/L)隐丹参酮对CD133^+肾癌干细胞凋亡的影响;免疫印迹检测5 mg/L隐丹参酮对肾癌干细胞中Ki67、Bcl-2、Caspase-3和p-Caspase-3蛋白表达的影响。结果与结论:分选前CD133^+阳性细胞的百分率为6.32%,分选后CD133^+阳性细胞的百分率为82.73%,差异有显著性意义(P<0.001)。隐丹参酮抑制肾癌干细胞生长,促进肾癌干细胞凋亡,并呈浓度依赖性。Ki67和Bcl-2在5 mg/L隐丹参酮组中的表达明显低于未加药组,p-Caspase-3的表达水平则明显高于未加药组,而Caspase-3在两组中的表达水平没有明显变化,结果表明隐丹参酮可能通过抑制肾癌干细胞Ki67和Bcl-2表达,促进p-Caspase-3表达来发挥抑增殖促凋亡效应。BACKGROUND：Previous studies have found that cryptotanshinone represses multiple tumors,but little is reported on its effect on renal carcinoma.OBJECTIVE：To explore the effect of cryptotanshinone on the proliferation and apoptosis of the renal carcinoma stem cells.METHODS：CD133~＋ renal carcinoma stem cells were separated from OS-RC-2 cells by immunomagnetic bead separation.Effects of 0,0.2,1,5 mg/L cryptotanshinone on the proliferation and apoptosis of CD133~＋ renal carcinoma stem cells were detected by MTT and flow cytometry,respectively.Expression levels of Ki67,Bcl-2,Caspase-3 and p-Caspase-3 protein were detected by western blot assay.RESULTS AND CONCLUSION：After magnetic cell sorting,the percentage of CD133~＋ cells was increased from6.32%to 82.73%,and there was a significant difference before and after cell sorting（P〈0.001）.Cryptotanshinone could repress the proliferation of CD133^＋ renal carcinoma stem cells and promote cell apoptosis in a dose-dependent manner.The protein expression levels of Ki67 and Bcl-2 in the 5 mg/L cryptotanshinone group were significantly decreased compared with the control group,while the protein expression level of p-Caspase-3 protein was significantly increased.In addition,there was no difference in the protein expression of Caspase-3 between cryptotanshinone and control group.These findings indicate that cryptotanshinone may be a potent anticancer drug for the treatment of renal carcinoma by inhibiting expression of Ki67 and Bcl-2 and promoting protein expression of p-Caspase-3.

关 键 词：肾肿瘤 肿瘤干细胞 丹参 细胞增殖 细胞凋亡 组织工程 干细胞 隐丹参酮 肾癌 肾癌干细胞 增殖 凋亡 

分 类 号：R394.2[医药卫生—医学遗传学]