内皮样细胞与脐静脉内皮细胞的比较  

Endothelial-like cells versus human umbilical vein endothelial cells

作　　者：郝晓娟[1] 郝海英[1] 朱敏杰[1] 袁铮[1] 李伟伟[1] 陈洁[1] 朱旅云[2] 

机构地区：[1]邯郸市中心医院肾内二科,河北省邯郸市056001 [2]白求恩国际和平医院,河北省石家庄市050000

出　　处：《中国组织工程研究》2016年第1期83-88,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:干细胞可诱导分化为内皮样细胞,用于治疗糖尿病下肢血管病变,但诱导后的内皮样细胞是否完全可以取代内皮细胞改善血管病变呢?内皮样细胞与内皮细胞有哪些异同?尚未得到解决。目的:探讨诱导后的内皮样细胞与脐静脉内皮细胞在形态、功能及活性方面的异同。方法:分离、培养脐带间充质干细胞及脐静脉内皮细胞,分别通过流式细胞仪及免疫组化进行鉴定,将分离出的脐带间充质干细胞用含10μg/L血管内皮生长因子、10μg/L的碱性成纤维细胞生长因子及体积分数为2%胎牛血清的DMEM-LG/F12培养液向内皮样细胞方向诱导分化并用免疫组化进行鉴定,通过细胞迁移、活性物质测定及三维血管形成实验对内皮样细胞及内皮细胞进行比较。结果与结论:分离的脐带间充质干细胞经流式及免疫组化鉴定高表达间充质干细胞相关表面标志,脐静脉内皮细胞经免疫组化鉴定高表达CD31和VWF。脐带间充质干细胞经诱导液诱导后,进行免疫组化鉴定高表达CD31和VWF。细胞迁移、活性物质及三维血管形成实验均显示诱导后的内皮样细胞在功能及活性上与内皮细胞相似,且优于内皮细胞。BACKGROUND： Stem cells are induced to differentiate into endothelial-like cells that can be used for the treatment of diabetic lower extremity vascular disease. However, it is unclear whether these endothelial-like cells can completely replace endothelial cells to improve vascular disease and what are the differences between endothelial-like cells and endothelial cells. OBJECTIVE： To explore the differences and similarities between endothelial-like cells and human umbilical vein endothelial cells in the aspects of morphology, function, and viability. METHODS： Umbilical cord mesenchymal stem cells and umbilical vein endothelial cells were isolated, cultured and identified using flow cytometry and immunohistochemical method. Isolated umbilical cord mesenchymal stem cells were induced in DMEM-LG/F12 containing 10 μg/L vascular endothelial growth factor, 10 μg/L basicfibroblast growth factor and 2% fetal bovine serum to differentiate into endothelial-like cells followed by immunohistochemical identification. To compare endothelial-like cells with human umbilical vein endothelial cells, cell migration detection, active substance measurement and three-dimensional angiogenesis test were performed. RESULTS AND CONCLUSION： Isolated umbilical cord mesenchymal stem cells strongly expressed the surface markers of mesenchymal stem cells, and human umbilical vein endothelial cells strongly expressed CD31 and VWF. After induction, the umbilical cord mesenchymal stem cells were identified to highly express CD31 and VWF. Through cell migration, active substance and three-dimensional angiogenesis tests, endothelial-like cells were similar to endothelial cells in the function and activity, and superior to endothelial cells.

关 键 词：脐带 间质干细胞 内皮细胞 细胞迁移分析 组织工程 干细胞 培养 脐带间充质干细胞 内皮样细胞 细胞迁移 血管形成 

分 类 号：R394.2[医药卫生—医学遗传学]