异氟醚对新生大鼠海马神经干细胞增殖及分化的影响  

Isoflurane effects on the proliferation and differentiation of neural stem cells in the hippocampus of neonatal rats

作　　者：闵娜[1] 胡强夫[1] 李晓培[1] 聂晓红[1] 杨利利[1] 

机构地区：[1]郑州大学第五附属医院麻醉科,河南省郑州市450052

出　　处：《中国组织工程研究》2016年第1期118-122,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:异氟醚麻醉药物会对神经系统产生一定的影响,研究表明其可能是通过影响神经干细胞功能或形态而引起神经功能障碍。目的:探讨异氟醚对大鼠海马神经干细胞增殖和分化的影响。方法:培养新生7 d SD大鼠海马神经干细胞并进行诱导分化,取传至第3代细胞,将其分为2组:异氟醚组通入2.8%异氟醚和体积分数为5%CO_2,体积分数为95%O_2的混合气体;对照组仅通入体积分数为5%CO_2,体积分数为95%O_2的混合气体。干预6 h后,再常规培养2 h。抗Brd U单克隆抗体进行免疫荧光染色检测细胞增殖情况,Western blot法测定神经元标志物β3-微管蛋白和胶质细胞标志物GFAP蛋白的表达。结果与结论:与对照组相比,异氟醚组Brd U阳性细胞数目明显减少,说明异氟烷抑制了神经干细胞增殖;与对照组相比,异氟醚组GFAP表达水平上调,β3-微管蛋白表达水平无明显改变,说明异氟烷可促进神经干细胞更多地向星形胶质细胞分化。BACKGROUND： Isoflurane is an anesthesia drug that has a certain effect on the nervous system. It possibly causes neurologic disorders through impacting nerve stem cell function or morphology. OBJECTIVE： To investigate the effects of isoflurane on the proliferation and differentiation of neural stem cells in the hippocampus of rats. METHODS： Neural stem cells from the hippocampus of neonatal Sprague-Dawley rats, aged 7 days, were induced and differentiated. Passage 3 cells were obtained and divided into two groups： isoflurane group（a mixture gas of 2.8% isoflurane, 5% CO_2 and 95% O_2） and control group（a mixture of 5% CO_2 and 95% O_2）. Afterintervention of 6 hours followed by 2 hours of routine culture, anti-Brd U monoclonal antibody immunofluorescent staining was used to detect cell proliferation, and western blot assay to detect the expression of β3-tubulin and glial fibrillary acidic protein. RESULTS AND CONCLUSION： Compared with the control group, the number of Brd U positive cells in the isoflurane group reduced significantly, indicating that isoflurane inhibits the proliferation of neural stem cells. Compared with the control group, the expression of glial fibrillary acidic protein in the isoflurane group up-regulated, but the expression of β3-tubulin had no changes, indicating isoflurane promotes the differentiation of neural stem cells into astrocytes.

关 键 词：神经干细胞 异氟醚 麻醉 吸入 细胞增殖 组织工程 干细胞 培养 吸入麻醉 大鼠 新生 海马 神经元分化 

分 类 号：R394.2[医药卫生—医学遗传学]