益气除痰方含药血清对顺铂耐药肺癌细胞增殖和细胞周期的影响  

Effects of Yiqi Chutan formula serum on proliferation and cell cycle of cisplatin-resistant lung cancer cells

作　　者：张恩欣[1] 周岱翰[1] 侯超[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405

出　　处：《山东医药》2016年第6期13-15,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81573918);广东省自然科学基金资助项目(2014A030313413)

摘　　要：目的观察益气除痰方食药血清对顺铂耐药肺癌细胞A549/DDP增殖和细胞周期的影响。方法制备益气除痰方含药血清和空白血清。分别用0(空白血清)、0.5%、1%、2.5%、5%、10%、20%、30%益气除痰方含药血清培养肺癌A549/DDP细胞,用MTT比色法测算增殖抑制率和半数抑制浓度(IC50)。用0、50%IC50、100%IC50浓度益气除痰方含药血清培养肺癌A549/DDP细胞,用流式细胞术测算G0/G1期、S期、G2/M期细胞比例。结果0.5%、1%、2.5%、5%、10%、20%、30%益气除痰方含药血清培养A549/DDP细胞48 h后,细胞增殖抑制率分别为5.80%±0.95%、9.97%±1.26%、27.10%±3.22%、43.03%±2.02%、73.70%±4.69%、86.13%±1.59%、91.77%±1.65%,随着益气除痰方含药血清浓度增加,A549/DDP细胞增殖抑制率升高(P均<0.05),益气除痰方含药血清对A549/DDP细胞的IC50为8.56%。与益气除痰方含药血清浓度为0者比较,50%IC50和100%IC50浓度益气除痰方含药血清培养的A549/DDP细胞G0/G1期细胞比例增加,S期和G2/M期细胞比例降低,且药物浓度越高变化越显著(P均<0.05)。结论益气除痰方含药血清可抑制顺铂耐药肺癌A549/DDP细胞增殖,这可能与其阻滞A549/DDP细胞从G1期进入S期有关。Objective To investigate the effects of Yiqi Chutan formula（ YQCTF） on proliferation and cell cycle of cisplatin-resistant human lung cancer cells A549 / DDP. Methods The YQCTF serum and blank serum was made. The YQCTF serum containing 0,0. 5%,1%,2. 5%,5%,10%,20% and 30% YQCTF was used to culture the cisplatin-resistant A549 / DDP cells. The proliferation inhibition rate and IC50 was evaluated by MTT assay. The YQCTF serum with the concentration of 0,50% IC50 and 100% IC50 were used to culture the cisplatin-resistant A549 / DDP cells,and the cell percentages in the G0/ G1 phase,S phase and G2/ M phase. Results The proliferation inhibition rates were respectively5. 80% ± 0. 95%,9. 97% ± 1. 26%,27. 10% ± 3. 22%,43. 03% ± 2. 02%,73. 70% ± 4. 69%,86. 13% ± 1. 59%and 91. 77% ± 1. 65% when the A549 / DDP cells were cultured with 0. 5%,1%,2. 5%,5%,10%,20% and 30%YQCTF serum for 48 h. The proliferation inhibition rate was increased with the increasing concentrations of YQCTF serum（ all P 0. 05）. IC50 was 8. 56%. The cell percentage of A549 / DDP cells in the G0/ G1 phase was increased when the A549 / DDP cells were cultured with the concentrations of 50% IC50 and 100% IC50 YQCTF serum as compared with 0 IC50 YQCTF serum. The percentage of A549 / DDP cells in the S phase and G2 / M phase was decreased,and the higher the concentrations were,the more significant the changes would be（ all P 0. 05）. Conclusion YQCTF serum could inhibit the proliferation of cisplatin-resistant human lung cancer cells A549 / DDP,which may be related with its function of blocking cells from G1 phase to S phase.

关 键 词：肺癌细胞 益气除痰方 顺铂耐药 细胞增殖 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤]