非小细胞肺癌组织中AKT1、PDCD4蛋白表达变化及意义  

Expression changes of AKT1 and PDCD4 in non-small-cell lung cancer tissues and the significance

作　　者：王嘉[1] 吕艳超[2] 许浩然[3] 韩双双[1] 朱振龙[1] 

机构地区：[1]河北医科大学第一医院,石家庄050031 [2]保定市第一中心医院总院 [3]承德市中心医院

出　　处：《山东医药》2016年第6期16-18,共3页Shandong Medical Journal

基　　金：河北省医学科学研究重点课题(20090333)

摘　　要：目的观察非小细胞肺癌(NSCLC)组织中丝/苏氨酸蛋白激酶1(AKT1)和程序性细胞死亡因子4(PDCD4)蛋白表达变化,并探讨其临床意义。方法收集65例NSCLC患者的手术切除肿瘤组织为NSCLC组,30例受检者的正常肺泡组织为肺泡对照组,30例受检者的正常支气管断端组织为支气管对照组。用免疫组化法检测三组标本中的AKT1、PDCD4,并分析NSCLC组二者表达与肿瘤临床病理参数的关系。结果与支气管对照组和肺泡对照组相比,NSCLC组AKT1表达升高,PDCD4表达降低,P均<0.05。NSCLC组AKT1表达与NSCLC分化程度、淋巴结转移及TNM分期有关(P均<0.05),与患者的性别、年龄和肿瘤的大小、组织学类型无关;NSCLC组中PDCD4表达与NSCLC组织学类型有关(P<0.05),与患者的性别、年龄和肿瘤的大小、分化程度、淋巴结转移及TNM分期无关;AKT1和PDCD4在肺腺癌组织中表达呈负相关(r=-0.436,P=0.021),在肺鳞癌组织中无关(r=-0.012,P=0.931)。结论 NSCLC组织中AKT1表达升高、PDCD4表达降低,联合检测两者有助于NSCLC的诊治和预后判断。Objective To observe the expression changes of AKT1 and programmed cell death receptor 4（ PDCD4）in tissues of non-small-cell lung cancer（ NSCLC） and to investigate its clinical significance. Methods We selected 65 cases of NSCLC tissues（ NSCLC group）,30 cases of normal alveolar tissues（ alveolar group） and 30 cases of bronchial stump tissues（ bronchial group）. The expression of AKT1 and PDCD4 in the three groups was detected by immunohistochemistry,and we analyzed its relationship with the clinical and pathological data in the NSCLC group. Results Compared with the alveolar group and bronchial group,the expression of AKT1 was increased while the expression of PDCD4 was decreased in the NSCLC group（ all P 0. 05）. In the NSCLC group,the expression of AKT1 was related with tumor differentiation,lymph node metastasis and TNM staging（ all P 0. 05）,but was not related with gender,age,tumor size and histological type; the expression of PDCD4 was related with tumor histological type（ P 0. 05）,but was not related with gender,age,tumor size,differentiation,lymph node metastasis and TNM staging. The expression of AKT1 and PDCD4 in the lung adenocarcinoma tissues was negatively correlated（ r =- 0. 436,P = 0. 021）,but was not related with each other in the lung squamous-cell carcinoma tissues（ r =- 0. 012,P = 0. 931）. Conclusion The expression of AKT1 was increased and PDCD4 was decreased in NSCLC tissues,and the combined detection of them contributed to the diagnosis,treatment and prognosis of NSCLC.

关 键 词：非小细胞肺癌 肺泡 支气管 丝/苏氨酸蛋白激酶1 程序性细胞死亡因子4 

分 类 号：R734.2[医药卫生—肿瘤]