双环己酮草酰二腙诱导小鼠髓鞘脱失的雌雄差异  

Difference of biscyclohexanone oxaldihydrazone-induced demyelination in female and male mice

作　　者：王娓娓[1] 鲍天昊[1,2] 杨飏[2] 张红苗[1] 王淑芬[3] 肖志成[3] 韩剑虹[1] 

机构地区：[1]昆明医科大学第二附属医院,昆明650101 [2]云南省精神病医院 [3]昆明医科大学

出　　处：《山东医药》2016年第6期33-35,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(31460314)

摘　　要：目的比较双环己酮草酰二腙诱导雌雄小鼠髓鞘脱失的差异。方法取雌雄小鼠各10只分别为雌、雄鼠组,均喂养含有0.2%双环己酮草酰二腙的饲料6周建立脱髓鞘模型。用转杆测试仪检测两组小鼠的运动平衡与协调能力,用LFB-PAS染色法观察两组小鼠胼胝体髓鞘脱失情况,用免疫荧光染色法检测两组小鼠脑组织切片髓鞘碱性蛋白(MBP)荧光强度,用Western blot法检测两组小鼠脑组织匀浆MBP水平。结果雌鼠组小鼠运动时间(117.60±18.48)s、掉落次数(3.2±0.8)次、髓鞘脱失程度评分(2.20±0.27)分、脑组织切片MBP荧光强度65.20±6.46、脑组织匀浆MBP水平0.44±0.06,雄鼠组分别为(90.80±8.70)s、(4.6±0.9)次、(1.70±0.27)分、47.31±5.79、0.27±0.05。与雌鼠组相比,雄鼠组运动时间较短,掉落次数较多,髓鞘评分较低,脑组织切片MBP荧光强度较低,脑组织匀浆MBP水平较低,P均<0.05。结论在双环己酮草酰二腙诱导的脱髓鞘小鼠模型中,雄鼠更容易脱髓鞘。Objective To compare the difference of biscyclohexanone oxaldihydrazone（ cuprizone）-induced demyelination in female and male mice. Methods Ten male mice and 10 female mice were selected and fed with 0. 2% cuprizone for 6 weeks in order to establish the demyelination model. Rotarod test was used to examining motor function of balance and coordination. Luxol fast blue-periodic acid Schiff（ LFB-PAS） staining was used to examine the area of demyelination in corpus callosum. The fluorescence intensity of myelin base protein（ MBP） in corpus callosum was examined by immunohistochemistry with antibody of MBP. Western blotting was performed to examining the levels of MBP in the brain tissues. Results In the female mice（ female group）,the running time was（ 117. 60 ± 18. 48） s,the times of falling off were 3. 2 ±0. 8,the myelination scores were 2. 20 ± 0. 27,the intensity of MBP was 65. 20 ± 6. 46 and the expression of MBP was0. 44 ± 0. 06; and those indexes in the male mice（ male group） were（ 90. 80 ± 8. 70） s,4. 6 ± 0. 9,1. 70 ± 0. 27,47. 31± 5. 79,and 0. 27 ± 0. 05. Compared with the female group,the running time was shorter,the times of falling off were more,the myelination score was lower,MBP intensity was lower,and the relative expression of MBP was lower in the male group（ all P 0. 05）. Conclusion In the cuprizone-induced demyelination models,the male mice are more easily to demyelination than the female mice.

关 键 词：多发性硬化 性别 髓鞘脱失 双环己酮草酰二腙 

分 类 号：R744.5[医药卫生—神经病学与精神病学]