非酒精性脂肪肝大鼠肝组织FGF21 mRNA表达与脂质沉积、胰岛素抵抗的关系  

Correlations of FGF21 mRNA expression with fat deposition and insulin resistance in rat liver with NAFLD

作　　者：申甜[1] 徐碧林[1] 盛霞[1] 高月锦[1] 汪红平[1] 喻明[1] 

机构地区：[1]上海中医药大学附属普陀医院,上海200062

出　　处：《山东医药》2016年第8期10-13,共4页Shandong Medical Journal

基　　金：上海市卫生局青年科研项目(2013Y079);上海市中医药大学后备业务专家培养计划

摘　　要：目的观察非酒精性脂肪肝(NAFLD)大鼠肝组织成纤维细胞生长因子21(FGF21)mRNA表达,并探讨其与脂质沉积、胰岛素抵抗的关系。方法选择SD大鼠40只,随机分为对照组10只、高脂组30只。对照组给予基础饲料喂养,高脂组给予高脂饲料喂养。喂养8周,两组均取10只,评价NAFLD模型建立情况。高脂组剩余大鼠继续高脂饲料喂养,分别于12、24周各取10只,腹主动脉取血,检测血清空腹血糖(FPG)、空腹胰岛素(FINS)、ALT、AST、TC、TG、FGF21、FFA水平,计算胰岛素抵抗指数(HOMA-IR);处死大鼠取肝组织,制备石蜡切片,行HE染色,观察肝细胞形态学变化;制备肝组织匀浆液,检测TG含量;采用RT-PCR法检测肝组织FGF21 mRNA表达。结果喂养8周时,高脂组体质量、肝湿重及血清TG、TC水平均高于对照组(P均<0.05),肝组织呈大泡性脂肪变性并出现炎症细胞浸润及点状坏死,提示NAFLD模型建立成功。高脂组喂养12、24周时,血清FFA水平、肝组织TG含量、HOMA-IR明显高于喂养8周时,且喂养24周时均高于喂养12周时(P均<0.05);而血清FGF21水平、肝组织FGF21 mRNA表达则呈先升高再下降趋势(P均<0.05)。结论 NAFLD大鼠肝组织FGF21 mRNA表达、血清FGF21水平与肝组织TG含量有关,在NAFLD早期二者变化一致,胰岛素抵抗可间接抑制其表达。Objective To observe the fibroblast growth factor 21（ FGF21） mRNA expression level in rat liver with non-alcoholic fatty liver disease（ NAFLD） and to investigate its relationship with fat deposition and insulin resistance.Methods Forty SD male rats were randomly divided into the control group（ n = 10,NC group） fed with basic food and high-fat group（ n = 30,HF group） fed with high-fat diet. After 8-week feeding,10 rats were selected to observe whether the NAFLD models were built successfully in the HF group. After that the rest of rats in the HF group were fed with highfat diet,and at week 12,10 rats were selected and at week 24,the other 10 were selected. The fasting blood-glucose（ FPG）,fasting insulin（ FINS）,alanine transamianse（ ALT）,aspartate transamianse（ AST）,total cholesterol（ TC）,triglyceride（ TG）,serum FGF21,serum FFA,and homeostasis model assessment of insulin resistance（ HOMA-IR） were calculated. The pathological change of liver was observed by HE staining; liver triglyceride（ TG） content was tested and the expression of FGF21 mRNA in liver was detected by RT-PCR. Results At the 8 th week,the body mass,liver-wet weight,serum TG and TC of the HF group were higher than those of the NC group（ all P 0. 05）,liver tissues of the HF group changed to bullous steatosis and had inflammatory cell infiltration and spotty necrosis,so that NAFLD model was built successfully. In the end of 12 and 24 th weeks,the serum FFA,TG content and HOMA-IR were significantly higher than those of the 8th week,and the 24 th week was also higher than the 12 th week（ all P 0. 05）. During the week 8-12,the serum FGF21 and expression of FGF21 mRNA in liver of the HF group increased first and then decreased（ all P 0. 05）.Conclusion The expression of FGF21 mRNA in liver and serum FGF21 of NAFLD rats were related with the liver TG content. Besides,both of them have the same change in the early stage of NAFLD,and insulin resistance may inhibit the expression of FGF21 mRNA indirect

关 键 词：非酒精性脂肪性肝病 成纤维细胞生长因子 脂质沉积 胰岛素抵抗 大鼠 

分 类 号：R575.5[医药卫生—消化系统]