康复新在巨噬细胞炎症反应中的抗炎作用及其机制  

Anti-inflammatory effect and mechanism of Kangfuxin in inflammatory response of macrophages

作　　者：汤雁利 张玉皓[1,2] 李罡[3] 李启艳[1,2] 

机构地区：[1]云南省第一人民医院,昆明650032 [2]昆明理工大学附属医院 [3]昆明医科大学附属口腔医院

出　　处：《山东医药》2016年第9期13-15,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81160134);云南省中青年学术和技术带头人后备人才资助项目(2012HB027);云南省医学学科带头人资助项目(D-201232)

摘　　要：目的观察康复新在巨噬细胞炎症反应中的抗炎作用,并探讨其可能的作用机制。方法将培养好的小鼠巨噬细胞RAW264.7随机分为6组:A组(加入DMSO)、B组(加入DMSO)、C组(加入炎症抑制药物BAY11-7082)、D组(加入1%康复新)、E组(加入0.1%康复新)、F组(加入0.01%康复新),药物干预后继续培养1 h,A组加DMEM培养液,其余各组加入LPS(100μg/L)诱导细胞产生炎症反应。造模后4、8 h收集细胞,用qRT-PCR法检测细胞中白细胞介素(IL)6、肿瘤坏死因子(TNF)α、前列腺素内过氧化物合酶(PTGS)2的mRNA表达。结果造模后4、8 h,与A组比较,B组IL-6、TNF-α、PTGS2的mRNA表达水平均升高(P均<0.05);与B组比较,C组IL-6、TNF-α、PTGS2的mRNA表达均降低(P均<0.05)。D组在收集细胞时即观察到细胞全部死亡。与B组比较,E、F组IL-6、TNF-α的mRNA表达升高,PTGS2的mRNA表达降低(P均<0.05);与C组比较,E、F组IL-6、TNF-α的mRNA表达差异有统计学意义(P均<0.05),而PTGS2的mRNA表达差异无统计学意义(P均>0.05)。康复新的浓度、作用时间对IL-6、TNF-α、PTGS2的mRNA表达无影响。结论康复新对巨噬细胞炎症反应有抑制作用,该作用不是通过抑制IL-6、TNF-α等致炎因子的表达,而是与降低PTGS2的表达有关。Objective To observe the anti-inflammatory effects of Kangfuxin in cell inflammation of macrophages and to discuss the possible mechanism. Methods The cultivated mouse macrophages RAW264. 7 were randomly divided into 6groups： group A（ added with DMSO）,group B（ added with DMSO）,group C（ added with anti-inflammation drug BAY11-7082）,group D（ added with 1% Kangfuxin）,group E（ added with 0. 1% Kangfuxin）,and group F（ added with0. 01% Kangfuxin）. We continued to cultivate for 1 h after drug intervention,group A was treaded with DMEM,the other groups were treated with LPS（ 100 μg / L） to induce cell inflammation. The cells were collected after treatment of 4 h and8 h,the mRNA expression levels of IL-6,TNF-α and prostaglandin-endoperoxide synthase（ PTGS2） were detected by qRT-PCR. Results Four hours and eight hours after modeling,compared with group A,the mRNA expression levels of IL-6,TNF-α and PTGS2 were increased in the group B（ all P〈0. 05）; compared with group B,the mRNA expression levels of IL-6,TNF-α and PTGS2 were decreased in the group C（ all P〈0. 05）. All cells were dead in the group D. Compared with group B,the mRNA expression of IL-6 and TNF-α was increased and the PTGS2 mRNA expression was decreased in the groups E and F（ all P〈0. 05）. Compared with group C,significant difference was found in the mRNA expression of IL-6 and TNF-α between groups E and F（ all P〈0. 05）,and no significant difference was found in the mRNA expression of PTGS2 between groups E and F（ all P〉0. 05）. The concentration and duration of Kangfuxin had no effect on the mRNA expression of IL-6,TNF-α and PTGS2. Conclusion Kangfuxin has inhibitory effect on the cell inflammatory reaction,and its mechanism may be not related with the inhibition of expression of inflammatory factors,such as IL-6 and TNF-α,but be related with the down-regulated PTGS2 expression.

关 键 词：炎症 康复新 抗炎 巨噬细胞 细胞炎症模型 

分 类 号：R364.5[医药卫生—病理学]