白肉灵芝水提物对H_2O_2诱导PC12细胞凋亡的保护作用  

Protective effects of aqueous extracts of Ganoderma leucocontextum on H_2O_2-induced apoptosis in PC12 cells

作　　者：熊川[1] 陈诚[2] 黄文丽[1] 李强[1] 刘宗敏[3] 郑林用[3] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,成都610061 [2]四川省农业科学院植物保护研究所,成都610066 [3]四川省农业科学院,成都610066

出　　处：《食品安全质量检测学报》2016年第2期662-668,共7页Journal of Food Safety and Quality

基　　金：国家食用菌产业技术体系毛木耳与药用菌栽培岗位(CARS-24);四川省科技支撑计划项目(2013NZ0015);中药资源系统研究与开发利用省部共建国家重点实验室培育基地开发研究基金;以精深加工为核心的食药用菌产业关键技术集成与产业化项目(成财教[2013]265号);四川省食用菌产业技术路线图的编制(2013ZR0140)~~

摘　　要：目的对采自四川省凉山州会东县的白肉灵芝进行种属鉴定及抗氧化功效评价。方法基于样本的ITS序列完成系统发育分析,采用H_2O_2处理PC12细胞建模,MTT法检测细胞存活率,验证白肉灵芝水提物对PC12细胞的保护作用。结果样本与白肉灵芝Ganoderma leucocontextum同属于一个分支,相似度98%。样本水提物能显著提高H_2O_2处理后的PC12细胞的存活率并降低Caspase-3活性。结论样本鉴定为白肉灵芝Ganoderma leucocontextum,对H_2O_2诱导的PC12细胞凋亡有保护作用,其机制可能与抑制细胞凋亡线粒体途径中Caspase-3的表达有关。Objective To identify the Ganoderma leucocontextum collected from Huidong county in Liangshan Yi Autonomous Prefecture, Sichuan, China and evaluate its antioxidant effect. Methods By DNA sequences of internal transcribed spacer（ITS）, the samples were identified. Apoptosis of PC12 cells were induced by H_2O_2, methyl thiazolyl tetrazolium（MTT） assay was used to detect the cell viability of PC12 cells protected by aqueous extracts of Ganoderma leucocontextum at different concentrations. Results The samples were identified as Ganoderma leucocontextum. Compared with the model group, aqueous extracts of Ganoderma leucocontextum could improved cell viability, decreased apoptotic cells and apoptosis rate, and decreased the expression of Caspase-3. Conclusion Aqueous extracts of Ganoderma leucocontextum can inhibit H_2O_2-induced apoptosis in PC12 cells, while the mechanism may be related to the inhibition of the expression of apoptosis-related protein Caspase-3 in the mitochondrial pathway.

关 键 词：白肉灵芝 系统发育关系 PC12细胞 抗氧化 

分 类 号：R285[医药卫生—中药学]