采前钙处理对黄金梨果顶硬化果实贮藏特性及PpEXPA2基因表达的影响  

Effects of Pre-harvest Calcium Treatment on the Storage Characteristics of Hard-end Whangkeumbae Pear Fruits and the Expression of PpEXPA2

作　　者：王玉玲[1] 路贵龙 张新富[1] 王然[1] 杨绍兰[1] 

机构地区：[1]青岛市园艺植物遗传改良与育种重点实验室青岛农业大学园艺学院,山东青岛266109

出　　处：《现代食品科技》2016年第2期53-59,120,共8页Modern Food Science and Technology

基　　金：国家自然科学基金项目(31201608);国家现代农业(梨)产业技术体系建设专项(nycytx-29-06);山东省优秀中青年科学家科研奖励基金(BS2010NY009);青岛市科技计划基础研究项目(12-1-4-5-(8)-jch);青岛农业大学高层次人才启动基金(630803)

摘　　要：为探究采前钙处理对黄金梨果顶硬化果实的影响,以黄金梨正常果实和果顶硬化果实为试材,对部分果顶硬化果实进行采前喷钙处理,分别测定果实的贮藏特性和PpEXPA2基因表达量等指标。结果表明:2℃贮藏期间,正常果实、CaCl2处理果实及果顶硬化果实萼端的硬度、粘性、弹性、内聚性和咀嚼性均呈先升高后降低趋势,CaCl2处理果实萼端及柄端的硬度和咀嚼性均明显低于果顶硬化果实;正常果实失重率最高,其次为CaCl2处理果实;CaCl2处理降低了果顶硬化果实的腐烂率,并抑制了果实的呼吸强度。贮藏180d,CaCl2处理果实的腐烂率和呼吸强度分别较果顶硬化果实降低了18.87%和59.70%;采收初期,PpEXPA2基因在果顶硬化果实中的表达明显高于正常果实和果顶硬化果实,随后下降,而正常果实和CaCl2处理果实中PpEXPA2基因的相对表达量均随贮藏时间逐渐升高,采前CaCl2处理抑制了该基因的表达。In order to investigate the influence of pre-harvest calcium treatment on the hard-end Whangkeumbae（Pyrus pyrifolia.Nakai.） pear fruits, normal fruits and hard-end fruits were used as test materials. Some of the hard-end pear fruits were sprayed with CaCl_2 solutions before harvest, and the fruit storage characteristics, gene expression level of PpEXPA2, and other indicators were determined. The results showed that during storage at 2 ℃, the firmness, adhesiveness, springiness, cohesiveness, and chewiness of the calyx-end area of normal fruit, CaCl_2-treated fruit and hard-end fruit showed a downward trend after the initial increase. The firmness and chewiness in both stem end and calyx end areas of CaCl_2-treated fruit were lower than those of hard-end fruit. The weight loss rate was highest in normal fruit, followed by in CaCl_2-treated fruit. CaCl_2 treatment reduced the rot rate of hard-end fruit and inhibited the respiration rate of the fruits. After 180 days of storage, the rot rate and respiration rate of CaCl_2-treated fruit were decreased by 18.87% and 59.70%, respectively, compared with hard-end fruit. During the early stage of the postharvest period, the relative expression level of Pp EXPA2 in hard-end fruit was significantly higher than those in normal fruit and CaCl_2-treated fruit and was then decreased. The relative expression levels of PpEXPA2 in normal fruit and CaCl_2-treated fruit gradually increased during the storage period. Pre-harvest CaCl_2 treatment may inhibit the gene expression of PpEXPA2.

关 键 词：黄金梨:果顶硬化 CACL2处理 贮藏特性 PpEXPA2 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]