固液态发酵条件对桑叶发酵提取物中降糖活性物质的影响  

Effect of Solid-state and Submerged Fermentation Conditions on Hypoglycemic Active Substances of Mulberry Leaf

作　　者：王吉成[1,2] 刘轩[2] 邹先伟[2] 程宣[2] 车冠达 李双双[2] 唐劲天[2] 翟延君[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]清华大学工程物理系,北京100084

出　　处：《现代生物医学进展》2016年第5期828-832,共5页Progress in Modern Biomedicine

基　　金：中国博士后科学基金特别资助(2014T70068)

摘　　要：目的:探讨不同发酵条件对桑叶总黄酮、总多酚含量及体外降糖活性的影响。方法:本研究主要利用比色法考察固态、液态两种发酵条件对桑叶发酵物中总黄酮、总多酚含量的影响,同时建立α-葡萄糖苷酶和α-淀粉酶为体外降糖活性筛选模型,以期筛选最佳发酵条件。结果:液体发酵7 d桑叶70%乙醇提取物对α-葡萄糖苷酶抑制率最高(92.79%);未发酵水提取物对α-淀粉酶抑制率最高(63.68%),其次为液体发酵7 d桑叶70%乙醇提取物(54.81%)。且在生药浓度为10 mg·m L-1时,都高于阳性对照阿卡波糖。结论:利用冠突散囊菌发酵,可以改变桑叶提取物的体外降糖活性,为开发糖尿病药物提供可行思路。Objective： To investigate the influence of different fermentation conditions on the total flavonoids content, total polyphenol content and vitro hypoglycemic activities from mulberry leaves. Methods： The influence of solid-state fermentation and submerged fermentation conditiongs was investigated for the total flavonoids content and tal polyphenol content of mulberry leaf extractions, and the vitro hypoglycemic active substance screening models of α-glucosidase and α-amylase were established so as to screen the best fermentation condition. Results： The 70% ethanol extraction by submerged fermented 7 d showed the highestα-glucosidase inhibitory activity which was 92.79%; and the water extraction of non-fermentation had higher α-amylase inhibitory activity which was 63.68% than the 70% ethanol extraction by submerged fermented 7 d which was 54.81%. At the same time, they were all higher than that of acarbose when the drug concentration was 10 mg·mL^-1. Conclusion： Fermentation by eurotim cristatum can change the vitro hypoglycemic activity of extract from mulberry leaves, which povides a feasible way for the development of drugs for diabetes.

关 键 词：桑叶 固体发酵 液体发酵 体外降糖 

分 类 号：R96[医药卫生—药理学] S567[医药卫生—药学]