GSRd对人脑胶质瘤U251细胞端粒酶活性和hTERT表达的影响  

Effects of Telomerase Activity and h TERT Gene Expression on Ginsenosides Rd in U251 Cells

作　　者：王莹[1] 宛蕾[1] 楚丽丽[1] 李建英[1] 祁冰洁[1] 

机构地区：[1]贵州医科大学基础医学院药理教研室,贵州贵阳550025

出　　处：《现代生物医学进展》2016年第5期857-859,共3页Progress in Modern Biomedicine

摘　　要：目的:探索人参苷皂Rd对人脑胶质瘤的作用。方法:人脑胶质瘤U251细胞系细胞培养,不同浓度的人参皂苷Rd处理观察细胞形态、测定端粒酶活性以及h TERT表达水平。结果:随着GSRd浓度的升高,U251细胞的生长被明显抑制,出现了细胞凋亡和凋亡小体的现象;在经GSRd处理后U251细胞的端粒酶活性,对照组和溶媒组类似,而GSRd药物处理24 h后,细胞端粒酶活性显著降低,其中20μg/L组端粒酶活性降低极显著,P<0.01,40μg/L和80μg/L组端粒酶活性显著降低,P<0.05。GSRd药物处理48 h后,细胞端粒酶在20、40、80μg/L处理后,端粒酶活性降低极显著,P<0.01;20μg/L、40μg/L和80μg/L的GSRd处理细胞后,相比于对照和溶媒组,h TERT基因表达水平显著降低。结论:人参皂苷Rd能够促进人脑胶质瘤U251细胞凋亡,对于临床治疗脑胶质瘤有重要的意义。Objective： To explore the role of ginsenosides Rd for human glioma. Methods： The human glioma cell line U251 cells were cultured, and the cell morphology, measurement of telomerase activity and h TERT expression levels were observed by by different concentrations of ginsenosides Rd treatment. Results： With GSRd concentration increased, the growth of U251 cells was significantly inhibited; in the U251 cells, telomerase activity similar to the control group and the vehicle treatment group after drug treatment with GSRd for 24 h, telomerase activity was significantly reduced, while 20 μg / L group telomerase activity decreased significantly, P〈0.01, telomerase activity lowered significantly in the 40 μg / L and 80 μg / L group, P〈0.05, P 0.05. GSRd drug treatment after 48 h, telomerase activity decreased significantly in the 20, 40, 80 μg / L group, P 0.01; Compared to the control and vehicle group, h TERT gene expression levels were significantly reduced in 20 μg / L, 40 μg / L and 80 μg / L group. Conclusion： Ginsenoside Rd can promote apoptosis in human U251 cells, it is important clinical significance for the treatment of glioma.

关 键 词：人参皂苷RD 人脑胶质瘤U251 端粒酶 h TERT 

分 类 号：R739.41[医药卫生—肿瘤]