白细胞介素-8对前列腺癌细胞增殖及迁移的影响  

The effect of IL-8 on prostate cancer cell proliferation and migration

作　　者：李鑫屹 杨杨[2] 黄校青[1] 曹宇[3] 李凤英 贾琳琳[1] 王丽敏[1] 陈洁茹[1] 赵晓莲[1] 王伟群[1] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007 [2]佳木斯大学临床医学院 [3]佳木斯大学第一附属医院

出　　处：《中国老年学杂志》2016年第6期1294-1296,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金面上资助项目(No.81272854);黑龙江省自然科学基金资助项目(No.D201129);黑龙江省大学生创新创业训练计划项目(No.201410222036);佳木斯大学研究生科技创新项目(No.LZR2014-004);佳木斯大学校长创新创业基金项目(No.xzyf 2014-12)

摘　　要：目的研究白细胞介素(IL)-8及其受体在前列腺细胞系的表达及IL-8对前列腺癌细胞增殖及迁移的影响。方法免疫荧光和Western印迹技术分别检测IL-8及其受体在前列腺细胞系中的表达;利用CCK8、克隆形成、划痕实验检测IL-8对前列腺癌细胞生长和迁移的影响。结果免疫荧光和Western印迹结果显示PC-3、DU145和RWPE-1细胞中都有IL-8及其受体的表达,PC-3细胞中IL-8及其受体表达明显多于DU145和RWPE-1(P<0.05);不同浓度的外源性IL-8能促进PC-3细胞增殖,随着浓度的增加细胞增殖效应也随之增加,浓度为80 ng/ml时增殖效果最明显,但浓度超过80 ng/ml后增殖效果有所下降,浓度为80 ng/ml时与其他组差异显著(P<0.05);克隆形成和划痕实验结果显示80 ng/ml的外源性IL-8能明显促进PC-3细胞克隆形成和迁移能力(P<0.01)。结论 IL-8可通过促进前列腺癌细胞生长、迁移而参与前列腺癌发生与演进过程。Objective To study the expression of interleukin（ IL）-8 and its receptor in prostate cancer cell lines and the effects of IL-8 on prostate cancer cell proliferation and migration. Methods The expression of IL-8 and its receptor were detected by immunofluorescence and Western blot,respectively; the proliferation and immigration were detected by CCK8,clone formation and scratch test. Results Immunofluorescence and Western blot showed that PC-3,DU145 and RWPE-1 expressed IL-8 and its receptor,the expression of IL-8 and its receptor in PC-3 cells were more than those of both DU145 and RWPE-1（ P 0. 05）. Different concentrations of IL-8 promoted proliferation of PC-3 cells,and the effect of proliferation increased gradually with the increase of concentration,the effect of proliferation was most obvious when the concentration was 80 ng / ml,however,the effect of proliferation would fall after concentration being more than 80 ng / ml,there was significant difference between 80 ng / ml of group and either of other groups（ P 0. 05）,the results of clone formation and scratches showed that 80 ng / ml of exogenous IL-8 obviously promoted clone formation and migration ability of PC-3 cells（ P 0. 01）. Conclusions IL-8 can participate in the occurrence and evolution of prostate cancer by promoting the growth and migration of prostate cancer cells.

关 键 词：白细胞介素(IL)-8 前列腺癌 增殖 迁移 

分 类 号：R737[医药卫生—肿瘤]