适度低氧微环境可促进人脑胶质瘤U251细胞体外生长  

Moderate hypoxic microenvironment promotes the growth of glioma U251 cells in vitro

作　　者：涂芸琥[1] 何永生[1,2] 吴波[1,2] 黄光富[2] 

机构地区：[1]遵义医学院,贵州遵义563003 [2]四川省医学科学院.四川省人民医院神经外科,四川成都610072

出　　处：《肿瘤》2016年第3期288-293,302,共7页Tumor

基　　金：2013年度中国科学院"西部之光"人才培养计划项目~~

摘　　要：目的 :探讨低氧微环境对人脑胶质瘤U251细胞体外生长、增殖、周期分布及凋亡的影响。方法 :将U251细胞置于不同氧浓度(常氧、3%氧浓度和1%氧浓度)条件下培养,然后倒置相差显微镜下观察各组细胞的形态差异,CCK-8法和平板克隆形成实验分别检测各组细胞的增殖活性及克隆形成能力,FCM法检测各组细胞的周期分布及凋亡情况。结果 :3%低氧组U251细胞较常氧组和1%低氧组生长状态更好。3%低氧组U251细胞的增殖活性和克隆形成能力均明显优于常氧组,而常氧组又优于1%低氧组,差异均具有统计学意义(P值均<0.05)。3%低氧组U251细胞中增殖期(S+G2)细胞所占比例明显高于常氧组,常氧组又高于1%低氧组(P值均<0.05);而3%低氧组细胞的凋亡率明显低于常氧组,常氧组又低于1%低氧组(P值均<0.05)。结论 :适度的低氧微环境能促进胶质瘤U251细胞的体外生长和增殖,提高增殖期细胞的比例,抑制细胞凋亡。Objective：To investigate the effect of hypoxic microenvironment on growth,proliferation,cell cycle distribution and apoptosis of human glioma U251 cells in vitro.Methods：The U251 cells were cultured under different concentrations of oxygen including normoxia and hypoxia（3%and 1%,respectively）.Then the cell morphology was observed by inverted phase contrast microscopy.The cell proliferation and colony-forming ability were measured by CCK-8 assay and colony formation assay,respectively.The cell cycle distribution and apoptosis were determined by FCM.Results：The U251 cells in 3%hypoxia group grew better than those in normoxia group and 1%hypoxia group.The proliferation activity and colony-forming ability of U251 cells in 3%hypoxia group were significantly better than those in normoxia group,while which in normoxia group were better than those in 1%hypoxia group（all P 〈 0.05）.The proportion of U251 cells at S phase and G2 phase in 3%hypoxia group was higher than that in normoxia group,while which in normoxia group was higher than that in 1%hypoxia group（both P 〈 0.05）.The apoptosis rate of U251 cells in 3%hypoxia group was lower than that in normoxia group,while which in normoxia group was lower than that in 1%hypoxia group（both P 〈 0.05）.Conclusion：Moderate hypoxic microenvironment（3%） may have potential to promote the growth and proliferation of glioma U251 cells,and increase the cell proportion at proliferative phase,as well as inhibit the process of apoptosis in vitro.

关 键 词：神经胶质瘤 细胞低氧 细胞增殖 细胞凋亡 细胞周期 

分 类 号：R739.41[医药卫生—肿瘤]