miR-221在早期食管鳞状细胞癌中高表达并促进食管鳞状细胞癌EC109细胞增殖、迁移和侵袭  

Up-regulation of miR-221 in early-stage esophageal squamous cell cancer and its promotion effect on proliferation, migration and invasion of EC109 cells

作　　者：郝志鹏[1] 唐朗[1] 邓豫[1] 蔡奕欣[1] 张霓[1] 付向宁[1] 李维娜[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院胸外科,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院感染科,湖北武汉430030

出　　处：《肿瘤》2016年第3期326-333,共8页Tumor

摘　　要：目的 :探讨微RNA(micro RNA,mi RNA,mi R)-221在早期食管鳞状细胞癌组织中的表达及其对食管鳞状细胞癌EC109细胞增殖、迁移和侵袭的影响。方法 :应用实时荧光定量PCR法检测42例早期食管鳞状细胞癌及其相应癌旁组织、食管鳞状细胞癌EC109细胞和人正常食管上皮HET-1A细胞中mi R-221的表达。分别将mi R-221模拟物(mi R-221 mimics)、模拟物阴性对照(mimics negative control,mimics NC)、mi R-221抑制物(mi R-221inhibitor)及抑制物阴性对照(inhibitor negative control,inhibitor NC)转染至EC109细胞后,应用实时荧光定量PCR法检测各组细胞中mi R-221的表达水平,CCK-8法、划痕愈合实验和Transwell小室法分别检测各组EC109细胞增殖、迁移和侵袭能力。结果 :早期食管鳞状细胞癌组织中mi R-221的表达水平显著高于相应癌旁组织(P<0.01),食管鳞状细胞癌EC109细胞中mi R-221的表达水平高于人正常食管上皮HET-1A细胞(P<0.05)。mi R-221 mimics转染后,EC109细胞中mi R-221的表达水平显著高于mimics NC转染组和未转染的空白对照组(P值均<0.01),细胞增殖(P值均<0.05)、迁移(P值均<0.01)和侵袭(P值均<0.01)能力均显著高于mimics NC转染组和未转染的空白对照组。mi R-221 inhibitor转染后,EC109细胞中mi R-221的表达水平显著低于inhibitor NC转染组和未转染的空白对照组(P值均<0.01),细胞增殖(P值均<0.05)、迁移(P值均<0.01)和侵袭(P值均<0.01)能力均显著低于inhibitor NC转染组和未转染的空白对照组。结论 :mi R-221在早期食管鳞状细胞癌组织中高表达,且能增强食管鳞状细胞癌EC109细胞体外增殖、迁移和侵袭能力。Objective：To investigate the expression of microRNA（miRNA,miR）-221 in early esophageal squamous cell cancer tissues and the effect of miR-221 on the abilities of proliferation,migration and invasion of esophageal squamous cell cancer ECl 09 cells.Methods：The expressions of miR-221 in 42 specimens of early-stage esophageal squamous cell cancer tissues and the corresponding para-cancerous tissues as well as in esophageal squamous cell cancer ECl 09 cells and esophageal epithelial HET-1A cells were detected by real-time fluorescent quantitative PCR.After transfection with miR-221 mimics,mimics negative control（mimics NC）,miR-221 inhibitor or inhibitor negative control（inhibitor NC）,respectively,the expression level of miR-221 in EC109 cells was detected by real-time fluorescent quantitative PCR.The abilities of cell proliferation,migration and invasion of ECl 09 cells were determined by CCK-8,wound healing and Transwell assay,respectively.Results：The expression level of miR-221 in early-stage esophageal squamous cell cancer tissues was significantly higher than that in the corresponding para-cancerous tissues（P 〈0.01）.The expression level of miR-221 in EC109 cells was higher than that in HET-1A cells（P 〈 0.05）.The expression level of miR-221 and abilities of cell proliferation,migration and invasion of ECl 09 cells after transfection with miR-221 mimics were significantly higher than those of ECl 09 cells transfected with mimics NC or without any transfection（as a blank control）（P 〈 0.01,P 〈 0.05,P 〈 0.01,P 〈 0.01,respectively）.However,the expression level of miR-221 and the abilities of cell proliferation,migration and invasion of EC109 cells after transfection with miR-221 inhibitor were significantly lower than those in EC109 cells transfected with inhibitor NC or without any transfection（P 〈 0.01,P 〈 0.05,P 〈 0.01,P 〈0.01,respectively）.Conclusion：miR-221 is highly expressed in early-stage esophageal squamous cell cancer tissues,and it can promote the ab

关 键 词：食管肿瘤 微RNAS 细胞增殖 细胞迁移 肿瘤浸润 MIR-221 

分 类 号：R735.1[医药卫生—肿瘤]