骨髓间充质干细胞对视网膜脱离兔神经营养因子表达的影响  

The effects of the bone marrow mesenchymal stem cells on the expression of neurotrophic factor protein gene in rabbits with retinal detachment

作　　者：冯程程[1] 邓爱军[2] 孙艳[2] 刘菲[3] 

机构地区：[1]潍坊医学院,261053 [2]潍坊医学院附属医院眼科,261031 [3]解放军第89医院眼科,潍坊261023

出　　处：《中华眼底病杂志》2016年第2期184-186,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的观察骨髓间充质干细胞（BMSC）对视网膜脱离（RD）后视网膜组织中神经营养因子表达的影响。方法健康白色家兔60只，随机分为正常对照组（A组）、RD＋磷酸盐缓冲液（PBS）组（B组）、RD＋BMSC组（c组），各组均为20只兔。B组、C组兔赤道部视网膜造孔建立RD模型。B组兔视网膜下腔注入PBS10pl；C组兔视网膜下腔注入CM-Dil标记的BMSCPBS悬液10μ1。A组兔不做任何处理。建模后1、2、4周，采用实时定量聚合酶链反应（RT—PCR）检测各组兔视网膜组织中碱性成纤维细胞生长因子（bFGF）、脑源性神经营养因子（BDNF）、睫状神经营养因子（CNTF）mRNA的表达。结果RT—PCR检测结果显示，1、2、4周时，C组兔视网膜组织中bFGF、BDNF、CNTFmRNA相对表达量均高于A组、B组，差异有统计学意义（P〈0．01）。1周时，B组兔视网膜组织中bFGF、CNTFmRNA相对表达量高于A组，BDNFmRNA相对表达量与c组基本相当。2周时，视网膜组织中bFGF、BDNFmRNA相对表达量呈低于A组，CNTFmRNA表达量高于A组；4周时，视网膜组织中bFGF、BDNF、CNTFmRNA相对表达量均低于A组。结论视网膜下腔移植BMSc可提高RD模型兔视网膜组织中bFGF、BDNF、CNTF基因表达。Objective To observe the effects of the bone marrow mesenchymal stem cells （BMSCs） on the expression of neurotrophic factor protein gene in the retinal detachment （RD） rabbits. Methods 60 healthy rabbits were randomly divided into control group （group A）, retinal detachment with PBS group （group B）, retinal detachment with BMSCs group （group C）, 20 rabbits in each group. RD model were established for rabbits in group B and C. 10 μl PBS was injected into the subretinal space of rabbits in group B, while 10 μl CM-Dil labeled BMSC PBS was injected into subretinal space of rabbits in group C. The rabbits in the group A received no treatment. At 1, 2 and 4 weeks after modeling, the mRNA expression of basic fibroblast growth factor （bFGF）, brain derived neurotrophic factor （BDNF） and ciliary neurotrophic factor （CNTF） were measured by real-time quantitative PCR. Results At 1, 2 and 4 weeks after modeling, the mRNA expression of bFGF, BDNF, CNTF on retinal tissue were increased significantly in group C as compared with group A and B （P〈0. 01）. At 1 week after modeling, the mRNA expression of bFGF and CNTF on retinal tissue were increased significantly in group B as compared with group A, the mRNA expression of BDNF on retinal tissue in group B was similar with group C. At 2 and 4 weeks after modeling, the mRNA expression of bFGF, BDNF, CNTF were decreased in group B as compared with group A. Conclusion Subretinal transplantation of BMSC can increase the mRNA expression of bFGF, BDNF and CNTF on retinal tissue in RD rabbits.

关 键 词：视网膜脱离/治疗 间质干细胞移植 神经生长因子类 动物实验 

分 类 号：R774.12[医药卫生—眼科]