基于SSR标记贵州古茶树资源的遗传多样性分析及指纹图谱构建  

Analysis of Genetic Diversity and Construction of Molecular Fingerprinting with SSR Markers for Ancient Tea Germplasms in Guizhou

作　　者：郭燕[1] 刘声传[1] 曹雨[1] 赵华富[1] 魏杰[1] 鄢东海[1] 周富裕[1] 

机构地区：[1]贵州省农业科学院茶叶研究所,贵州贵阳550006

出　　处：《西南农业学报》2016年第3期491-497,共7页Southwest China Journal of Agricultural Sciences

基　　金：贵州省科学技术基金项目"贵州古茶树种质资源ISSR分子标记研究"[黔科合J字(2012)2203];贵州省科研机构服务行动计划项目"黔茶系列茶树品种推广应用园区能力建设"[黔科合服企(2014)4008];贵州省农业公关项目"贵定鸟王茶种质资源的保护及开发利用"[黔科合NY字[2011]3046];贵州省农业动植物育种专项"黔茶1号区域适应性高效栽培研究与示范"[黔农育专字(2012)022]和"贵定鸟王种扩繁和栽培技术集成与示范"[黔农育专字(2015)003]

摘　　要：为了贵州古茶树资源的有效保护和充分利用提供理论依据,采用EST-SSR标记对40份贵州古茶树资源进行了遗传多样性分析和分子指纹鉴定。结果表明：20个SSR标记在40份资源中共检测到76个等位基因,平均每个标记3.8个,PIC和Shannon信息指数平均值分别为0.462和0.936,多态性适中。76个等位基因在参试古茶树资源中的出现频率差异较大,变异范围为1.32%-89.74%。参试材料的Nei＇s遗传距离（D）为0.075-0.875,当D≈0.160时,40份古茶树资源可聚为6类,包括3个单独聚类和3个复合聚类。利用4个核心标记即可鉴定40份古茶树资源,并根据等位基因带型,获得18位数的分子指纹图谱号码,构建每份资源的分子指纹图谱。In order to effectively conserve and reasonably utilize the ancient tea germplasms in Guizhou, the genetic diversity and molecular fingerprinting of 40 ancient tea plants, collected from the various ecological areas of Guizhou, were analyzed using EST-SSR marker. The results showed that total 76 alleles were found using 20 SSR markers with a mean value of 3.8. The average values of PIC and Shannon＇ s information index were 0.462 and 0.936 respectively, indicating the tested germplasms with moderate level of diversity. The occurrence frequency of 76 alleles differed largely, and it ranged from 3.57 % to 89.29 %. The Nei＇s genetic distance （D） of tested germplasms varied from 0. 075 to 0. 875. These germplasms at the D value of 0.16 were clustered into six categories, which included three single categories and three multiple categories. All tested ancient tea plants were identified by four core markers, and the molecular fingerprinting of each ancient tea plant was constructed using the 18-digit number obtained from allele band type.

关 键 词：古茶树资源 SSR 遗传多样性 指纹图谱 贵州 

分 类 号：S571.1[农业科学—茶叶生产加工]