卵清蛋白致敏小鼠嗜酸细胞性胃肠炎模型的制备  

Establishment of a mouse model of eosinophilic gastroenteritis induced by ovalbumin

作　　者：曾永梅[1] 杨素芳 钟婉莹 耿岚岚 杨敏 陈佩瑜 张纪泳[1] 龚四堂 

机构地区：[1]深圳市妇幼保健院,广东深圳518000 [2]广州市妇女儿童医疗中心,广东广州510120

出　　处：《临床儿科杂志》2016年第4期303-306,共4页Journal of Clinical Pediatrics

基　　金：广东省医学科研基金(No.B2015043)

摘　　要：目的 建立卵清蛋白致敏小鼠嗜酸细胞性胃肠炎模型.方法 6周龄BALB/c 雌性小鼠20 只,随机分为对照组及模型组.第1 天模型组用卵清蛋白（OVA）与氢氧化铝[AL（OH）3]凝胶腹腔注射基础致敏,第15 天模型组用OVA/ 生理盐水腹腔注射强化致敏,对照组均用等量生理盐水腹腔注射;第18 - 30天模型组隔日用聚乳酸-羟基乙酸共聚物（PLGA）包裹的OVA与生理盐水混匀灌胃激发,共7 次,对照组用等量生理盐水灌胃.造模后检测粪便隐血,计算活动指数评分（DAI）;第31 天处死小鼠,检测血清嗜酸性粒细胞（EOS）,分别取胃、回肠、结肠等组织,光镜下观察组织病理变化及EOS密度.结果 模型组小鼠在灌胃激发后均出现竖毛、弓背、懒动等表现,两组体质量变化无统计学差异,模型组DAI评分较对照组明显增高,差异有统计学意义（P 〈 0.01）;两组外周血EOS计数差异无统计学意义（P 〉 0.05）;光镜下胃肠黏膜EOS密度模型组较对照组增高,差异有统计学意义（P 〈 0.05）;光镜下模型组小鼠胃肠黏膜损伤明显.结论 致敏原OVA联合免疫佐剂氢氧化铝通过腹腔注射基础致敏及强化致敏,然后用PLGA包裹的OVA颗粒连续隔日灌胃7 次作激发,可以成功制备嗜酸细胞性胃肠炎小鼠动物模型.Objective To create a mouse model of eosinophilic gastroenteritis induced by ovalbumin. Methods Twenty6-week-old female BALB/c mice were randomly divided into experiment and control groups. The experiment group wasintraperitoneally injected with ovalbumin （OVA） and Al（OH）3 as basic sensitization on day 1, and then intraperitoneally injectedwith OVA and normal saline as strengthening sensitization on day 15. The control group was intraperitoneally injected thesame volume of normal saline. During days 18 to 30, the experiment group was challenged 7 times by intragastric injectionof OVA encapsulated by PLGA and normal saline every other day . The control group was intragastrically injected with samevolume of normal saline. During the molding period, fecal occult blood was detected and activity index score was counted.On day 31, all mice were sacrificed. The serum eosinophils （EOS） was detected. The tissues of stomach, ileum and colon weretaken to evaluate the pathological changes and EOS density under light microscope. Results Mice in the experiment groupshowed piloerection, roachback and lazy. The rat’s weigh was not different between two groups. The score of DAI was higherin experiment group than that in control group （P 〈 0.01）. The EOS count in peripheral blood was not different between twogroups （P 〉 0.05）. Under light microscope, the density of ESO in gastrointestinal mucosa was higher in experiment group thanthat in control group （P 〈 0.05）. The injury was more severe in gastrointestinal mucosa in experiment group than that in controlgroup. Conclusion Intraperitoneal injection of OVA and Al（OH）3 and then being challenged seven times by gavage with OVAwrapped by PLGA every other day, could successfully create a eosinophillic gastroenteritis model.

关 键 词：嗜酸细胞 胃肠炎 小鼠 

分 类 号：R-332[医药卫生] R725.7