CisoDGR多肽对乳腺癌MCF-7细胞增殖、凋亡的影响及其机制探讨  

Mechanism of cell proliferation and apoptosis of the breast cancer cell strain MCF-7 induced by synthesized polypeptides CisoDGR

作　　者：李旭[1,2] 黄尚科 安改丽[3] 韩瑞[2] 廖子君 赵新汉[2] 

机构地区：[1]陕西省肿瘤医院内一科,陕西西安710061 [2]西安交通大学医学院第一附属医院肿瘤内科,陕西西安710061 [3]陕西省人民医院肿瘤内科,陕西西安710068

出　　处：《现代肿瘤医学》2016年第9期1340-1344,共5页Journal of Modern Oncology

基　　金：国家自然科学基金面上项目(编号:30772112)

摘　　要：目的:探讨CisoDGR多肽对乳腺癌MFC-7细胞增殖和凋亡的影响及可能机制。方法:采用MTT法检测CisoDGR多肽对乳腺癌MCF-7细胞的抑制率;流式细胞术检测CisoDGR多肽对MCF-7细胞凋亡的影响;Western blot法检测Caspase-3、Bcl-2蛋白的表达。结果:CisoDGR多肽能明显抑制乳腺癌MCF-7细胞的增殖,诱导细胞凋亡,并存在一定的时-效及量-效关系。其对凋亡的诱导作用可能与Caspase-3蛋白表达增加及Bcl-2蛋白表达下降有关。结论:CisoDGR多肽具有抑制MCF-7细胞增殖,诱导MCF-7细胞凋亡的作用,其作用机制可能与Caspase-3、Bcl-2蛋白的表达变化有关。Objective：To investigate the effect of CisoDGR polypeptides on proliferation and apoptosis of human breast cancer MCF -7 cells as well as the relevant mechanism. Methods：MCF -7 cells were tested for proliferation activity by MTT assay, and analyzed for apoptosis rate and cell cycle by flow cytometry. The expressions of Caspase - 3 and Bcl- 2 were detected by Western blot. Results： CisoDGR polypeptides inhibited the cell proliferation and induced apoptosis in MCF - 7 cells in time - and dose - dependent way. The expression level of Bcl - 2 in MCF - 7 cells after treatment with CisoDGR polypeptides decreased significantly, while the level of Caspase - 3 was raised. Conclusion：CisoDGR inhibited the proliferation and promoted the apoptosis of MCF -7 cells by a mechanism which might be related to the changes of expressions of Caspase -3 and Bcl -2.

关 键 词：CisoDGR MCF-7 细胞增殖 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]