miR-92a调节RECK表达对髓母细胞瘤细胞增殖和迁移的影响及其机制  

Effect of miR-92a on the proliferation and migration of Daoy cells through RECK protein

作　　者：李楠[1] 李旭 史航宇[1] 高璐[1] 赵凌宇[3] 张刚[1] 史永强[1] 米伟阳 江彬[1] 希敏 

机构地区：[1]西安市儿童医院神经外科,陕西西安710043 [2]陕西省肿瘤医院内一科,陕西西安710061 [3]西安交通大学医学院医学部,陕西西安710061

出　　处：《现代肿瘤医学》2016年第9期1345-1348,共4页Journal of Modern Oncology

基　　金：陕西省自然科学基础研究计划项目(编号:2014JM4122)

摘　　要：目的：探讨miR-92a调节RECK表达对髓母细胞瘤细胞（Daoy）增殖迁移的影响并研究其可能的分子机制。方法：脂质体将miR-92a前体和阴性对照转染Daoy细胞,重组腺病毒介导RECK在Daoy细胞的表达,采用Taqman试剂盒检测miR-92a的表达,RealtimePCR检测转染后细胞中RECK等基因的表达,West-ernblot检测细胞中RECK等蛋白的表达,MTT检测细胞增殖能力,Transwell检测细胞侵袭迁移能力。结果：Daoy细胞中miR-92a表达高于miR-17~92基因簇的其他成员。过表达miR-92a增加Daoy细胞的增殖和迁移能力;miR-92a的过表达抑制RECK的蛋白表达;而腺病毒介导的RECK的过表达,则可以抑制Daoy细胞的增殖和迁移。结论：miR-92a可能通过降低RECK的表达来提高Daoy细胞的增殖和迁移能力。Objective：To investigate the effect of miR -92a on the proliferation and migration of Daoy cells and the possible mechanism. Methods：Synthetic miR -92a mimic and its negative control were transfected into Daoy cells by liposome method. Daoy cells were also infected with adenovirus encoding human RECK. After the treatment, the expression of microRNA was detected with Taqman microRNA kit. The mRNA level of target genes was quantified by Real time PCR and the protein level was detected with Western blot. The proliferation ability of Daoy cells was meas- ured with MTT and the migration ability was analyzed by Transwell. Results：miR -92a was relatively higher in Daoy cells compared to the other members in miR - 17 - 92 cluster. Overexpression of miR - 92a increased cell proliferation and migration in Daoy cells. RECK protein level was found decreased by miR -92a mimic. And adenovirus mediated RECK overexpression, in the other way, inhibit the proliferation and migration of Daoy cells. Conclusion： miR - 92a can increase proliferation and migration of Daoy cells, it may be achieved by down - regulating RECK protein expression.

关 键 词：人髓母细胞瘤 miR-92a RECK 

分 类 号：R739.41[医药卫生—肿瘤]