过表达CHD5基因对肝癌细胞SMMC-7721生物学行为的影响  

Effects of CHD5 on cells biological behavior in the hepatocellular carcinoma cell line SMMC-7721

作　　者：王继元[1] 费建平[1] 陆艺[1] 姜保成[1] 

机构地区：[1]上海交通大学医学院附属苏州九龙医院普外科,江苏苏州215000

出　　处：《现代肿瘤医学》2016年第9期1357-1360,共4页Journal of Modern Oncology

摘　　要：目的:探讨过表达CHD5基因对人肝癌细胞SMMC-7721生物学行为的影响。方法:采用脂质体法将CHD5过表达质粒及对照质粒分别转染SMMC-7721细胞,Western blotting检测SMMC-7721细胞中CHD5蛋白的表达情况,CCK-8法检测SMMC-7721细胞生长增殖能力的变化,流式细胞仪检测细胞凋亡,Transwell实验检测细胞迁移和侵袭能力的变化。结果:转染CHD5过表达质粒后,SMMC-7721细胞CHD5蛋白表达水平显著增高(P<0.01),抑制了细胞增殖、迁移和侵袭(P<0.05),促进了细胞凋亡(P<0.05)。结论:过表达CHD5基因可抑制SMMC-7721细胞的增殖、迁移和侵袭并诱导其凋亡。Objective：To explore the effect of CHD5 on cells biological behavior in hepatocellular carcinoma cell line SMMC - 7721. Methods：SMMC - 7721 cells were transfected with pcDNA3.1 （ - ）/CHD5 or negative control, Western blotting was performed to measure the expression of CHD5 protein, cell proliferation, apoptosis, migration and invasion was determined by CCK- 8 assay,flow cytometry and transwell assay, respectively. Results： CHD5 was upregulated after transfected with pcDNA3.1 （ - ）/CHD5 in SMMC -7721 cells compared with NC（ P 〈 0.01 ） ,inhibi- ted cells proliferation （ P 〈 0.05 ）, increased cells apoptosis （ P 〈 0.05 ）, and the ability of invasion and migration was significantly increased（P 〈 0.05）. Conclusion： Overexpression of CHD5 in SMMC -7721 cells may suppress cells proliferation, invasion and migration, induce cells apoptosis.

关 键 词：CHD5 肝癌细胞SMMC-7721 细胞增殖 细胞凋亡 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]