川芎嗪对人肺腺癌A549细胞增殖、迁移和侵袭的作用与机制  

Effects of Tetramethylpyrazine on proliferation,migration and invasion of lung cancer cell line A549

作　　者：牛媛[1] 王成[2] 张晓燕[1] 杨爱洁[2] 于洁[2] 阎超[2] 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]山东大学齐鲁医院(青岛)放疗科,山东青岛266035

出　　处：《现代肿瘤医学》2016年第10期1509-1512,共4页Journal of Modern Oncology

基　　金：山东省中青年科学家科研奖励基金(编号:BS2010YY014)

摘　　要：目的:探讨川芎嗪(tetramethylpyrazine,TMP)对人肺腺癌A549细胞增殖及侵袭的影响。方法:川芎嗪治疗组分别用0.1μg/ml、1μg/ml、10μg/ml、100μg/ml、250μg/ml浓度的川芎嗪干预A549细胞,同时设无药的阴性对照组及加顺铂的阳性对照组。24、48、72、96小时后,MTT法检测川芎嗪对A549细胞株增殖的抑制作用,划痕实验和Transwell法观测川芎嗪对A549细胞株迁移侵袭能力的影响,采用细胞流式术检测川芎嗪对A549细胞周期和凋亡的干预作用。结果:川芎嗪显著抑制A549细胞的增殖,抑制A549的迁移和侵袭,流式细胞术显示川芎嗪诱导A549细胞发生G1期阻滞和早期凋亡(P<0.05)。结论:川芎嗪对非小细胞肺癌A549细胞的抑制作用可能是通过诱导G1期细胞阻滞和早期凋亡引起的。Objective：To investigate the effect of tetramethylpyrazine （TMP） on the proliferation and invasion of human lung adenocarcinoma cell line A549. Methods：TMP treatment groups were treated with 0.1 μg/ml, 1 μg/ml, 10μg/ml,100μg/ml,250μg/ml TMP, and set up drug-reee negative control group and positive control group plus cisplatin. 24,48,72,96 hours later, the inhibitory effect of TMP on the proliferation of A549 cell line were detected by MTT method. The effects of TMP on the migration and invasion ability of A549 cell line were observed by scratch test and Transwell assay, and cell cycle and apoptosis were detected by cell flow cytometry. Results：TMP significantly inhibited the proliferation of A549 cells and inhibited migration and invasion of A549, flow cytometry showed TMP induced G1 arrest and early apoptosis in A549 cells （ P 〈 0.05 ）. Conclusion ： The inhibitory effect of TMP on A549 cells in nonsmall cell lung cancer may be induced by the G1 phase arrest and early apoptosis.

关 键 词：川芎嗪 A549 增殖抑制率 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]